GFP Reconstitution Across Synaptic Partners (GRASP) Methods to Dissect Mammalian

Project: Research project

Project Details

Description

DESCRIPTION (provided by applicant): GRASP (GFP Reconstitution Across Synaptic Partners), originally developed in invertebrates, is a genetically controllable fluorescence-based system for identifying sites of contact between two cells (or cell populations). It relies on the expression of membrane-tethered split (i.e. non-functional) GFP modules that must come together to reconstitute GFP fluorescence (spGFP1-10 & spGFP11); since this functional complementation requires close apposition between membranes (
StatusFinished
Effective start/end date9/1/115/31/16

Funding

  • National Institute of Neurological Disorders and Stroke: US$350,000.00
  • National Institute of Neurological Disorders and Stroke: US$346,501.00
  • National Institute of Neurological Disorders and Stroke: US$337,750.00
  • National Institute of Neurological Disorders and Stroke: US$350,000.00
  • National Institute of Neurological Disorders and Stroke: US$350,000.00

ASJC Scopus Subject Areas

  • Genetics

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