Mouse Metabolic Measurement System

Mouse Metabolic Phenotyping System Abstract Metabolic assessment is central to the study of many murine models of disease including classic metabolic disorders diabetes, obesity and anorexia, but metabolic profiling has increasing proven important in the study of atherosclerosis, fatty liver disease, cancer and more recently in the study of autoimmune disorders, aging and development. Columbia University Irving Medical Center (CUIMC) has been a leading center for the study of metabolism in murine models of disease and physiology for more two decades. The success of the research efforts by Columbia faculty has been anchored by the building and maintenance of a scientific infrastructure that permits state-of-the art assessment of metabolic phenotypes of mice by scientists with widely varying areas of focus, expertise and resources. The Naomi Berrie Diabetes Center has been central in building this infrastructure and NIH-funded programs in Diabetes, Cancer and Obesity have helped staff and maintain the varied facilities. This application proposes to maintain that infrastructure and grow its capabilities with the purchase of a Sable Promethion Metabolic Phenotyping System for the study of mice. The system will permit high temporal resolution in assessment of metabolic function (O2 consumption and CO2 production), motion, water intake, movement, feeding behavior, body weight and with additional sensors body temperature and continuous glucose monitoring. The 16 cage system will provide state-of-the-art technology in an integrated format with flexibility that is not offered by other systems. The system will be overseen by Dr. Anthony Ferrante and be incorporated into the existing infrastructure supported by the Naomi Berrie, the Columbia Diabetes and the NY Nutrition Obesity Research Centers. It will replace an antiquated system that currently is not operating and will expand the ability of a large group of productive Columbia investigators to complete funded proposals and provide new opportunities for further metabolic studies.