Structural Studies of Macromolecular Assemblies Using Cryo-EM

Project Narrative Single-particle cryo-EM, a method of molecular structure research pioneered in the PI's lab, has been further developed with the inclusion of time-resolved techniques to allow capture and structure determination of short- lived states. A combination of standard and time-resolved techniques will be applied to elucidate processes in protein biosynthesis with fundamental significance to public health. In addition, a novel method of data analysis aiming at characterization of the dynamic behavior of molecules will be applied to large datasets of important membrane-associated molecules: rotary ATPase and Cystic fibrosis trans-membrane conductance regulator.