Transcriptomics and Repertoire Profiling

  • Sims, Peter Alan (PI)
  • Sims, Peter P.A (PI)

Project: Research project

Project Details

Description

CORE C: PROJECT SUMMARY The main goal of this core is to establish and provide robust experimental procedures for profiling the transcriptomes and immune repertoires of tissue-resident lymphocytes. We will employ innovative methods for whole transcriptome and immune repertoire profiling in bulk populations, epigenomics, and single cell analysis. For low-input, population-level RNA-Seq we will use our recently established protocol for high-sensitivity mRNA capture and library construction. We will also provide bulk ATAC-Seq for measuring open chromatin. For single- cell RNA-Seq, we will deploy our microfluidic system for large-scale single cell capture and cDNA barcoding as well as a highly efficient plate-based method for whole transcriptome amplification. Finally, for dissecting the clonal architecture of tissue-resident T and B cells, we will provide cutting-edge methods for T cell receptor and antibody repertoire profiling.
StatusFinished
Effective start/end date6/25/185/31/23

Funding

  • National Institute of Allergy and Infectious Diseases: US$868,400.00
  • National Institute of Allergy and Infectious Diseases: US$481,750.00
  • National Institute of Allergy and Infectious Diseases: US$481,750.00
  • National Institute of Allergy and Infectious Diseases: US$855,047.00

ASJC Scopus Subject Areas

  • Genetics
  • Molecular Biology

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