Projects per year
Project Details
Description
CORE C: PROJECT SUMMARY
The main goal of this core is to establish and provide robust experimental procedures for profiling the
transcriptomes and immune repertoires of tissue-resident lymphocytes. We will employ innovative methods for
whole transcriptome and immune repertoire profiling in bulk populations, epigenomics, and single cell analysis.
For low-input, population-level RNA-Seq we will use our recently established protocol for high-sensitivity mRNA
capture and library construction. We will also provide bulk ATAC-Seq for measuring open chromatin. For single-
cell RNA-Seq, we will deploy our microfluidic system for large-scale single cell capture and cDNA barcoding as
well as a highly efficient plate-based method for whole transcriptome amplification. Finally, for dissecting the
clonal architecture of tissue-resident T and B cells, we will provide cutting-edge methods for T cell receptor and
antibody repertoire profiling.
Status | Finished |
---|---|
Effective start/end date | 6/25/18 → 5/31/23 |
Funding
- National Institute of Allergy and Infectious Diseases: US$868,400.00
- National Institute of Allergy and Infectious Diseases: US$481,750.00
- National Institute of Allergy and Infectious Diseases: US$481,750.00
ASJC Scopus Subject Areas
- Genetics
- Molecular Biology
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Projects
- 1 Finished
-
Tissue compartmentalization of human lymphocytes
Farber, D. (PI), Artis, D. (CoPI), Shen, Y. (CoPI), Shlomchik, M. (CoPI), Sims, P. A. (CoPI) & Sykes, M. (CoPI)
National Institute of Allergy and Infectious Diseases
6/1/13 → 5/31/22
Project: Research project