Abstract
Purpose: To develop a clinically relevant, robust murine model of rotator cuff tendon repair to examine cellular and molecular mechanisms of healing. Methods: Sixty C57BL/6 male mice underwent rotator cuff transection and repair using microsurgical techniques. A modified Kessler suturing technique was used prior to tendon detachment. Sutures were passed through 2 intersecting bone tunnels that were made at the tendon attachment site. Mice were sacrificed at 2 and 4 weeks with subsequent biomechanical, histologic, micro-CT, and gene expression evaluations. Results: Failure forces in the 2- and 4-week groups were 36% and 75% of the intact tendon, respectively. Histologic evaluation revealed complete reattachment of the tendon with no observable gap. Healing occurred by formation of fibrovascular tissue at the tendon-bone interface, similar to larger animal models. Molecular analysis revealed gene expression consistent with gradual healing of the reattached tendon over a period of 4 weeks. Comparisons were made using 1-way analysis of variance. Conclusions: This model is distinguished by use of microsurgical suturing techniques, which provides a robust, reproducible, and economic animal model to study various aspects of rotator cuff pathology. Clinical Relevance: Improvement of clinical outcomes of rotator cuff pathology requires in-depth understanding of the underlying cellular and molecular mechanisms of healing. This study presents a robust murine model of supraspinatus repair to serve as a standard research tool for basic and translational investigations into signaling pathways, gene expression, and the effect of biologic augmentation approaches.
Original language | English |
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Pages (from-to) | 1173-1183 |
Number of pages | 11 |
Journal | Arthroscopy - Journal of Arthroscopic and Related Surgery |
Volume | 34 |
Issue number | 4 |
DOIs | |
Publication status | Published - Apr 2018 |
Bibliographical note
Publisher Copyright:© 2017 Arthroscopy Association of North America
Funding
Funders | Funder number |
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National Institute of Arthritis and Musculoskeletal and Skin Diseases | T32AR007281 |
ASJC Scopus Subject Areas
- Orthopedics and Sports Medicine