Correlation of meniscal T2* with multiphoton microscopy, and change of articular cartilage T2 in an ovine model of meniscal repair

M. F. Koff; P. Shah; S. Pownder; B. Romero; R. Williams; S. Gilbert; S. Maher; L. A. Fortier; S. A. Rodeo; H. G. Potter

doi:10.1016/j.joca.2013.04.020

Correlation of meniscal T2* with multiphoton microscopy, and change of articular cartilage T2 in an ovine model of meniscal repair

M. F. Koff, P. Shah, S. Pownder, B. Romero, R. Williams, S. Gilbert, S. Maher, L. A. Fortier, S. A. Rodeo, H. G. Potter

Hospital for Special Surgery

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

Objective: To correlate meniscal T2* relaxation times using ultra-short echo time (UTE) magnetic resonance imaging (MRI) with quantitative microscopic methods, and to determine the effect of meniscal repair on post-operative cartilage T2 values. Design: A medial meniscal tear was created and repaired in the anterior horn of one limb of 28 crossbred mature ewes. MR scans for morphological evaluation, meniscal T2* values, and cartilage T2 values were acquired at 0, 4 and 8 months post-operatively for the Tear and Non-Op limb. Samples of menisci from both limbs were analyzed using multiphoton microscopy (MPM) analysis and biomechanical testing. Results: Significantly prolonged meniscal T2* values were found in repaired limbs than in control limbs, P<0.0001. No regional differences of T2* were detected for either the repaired or control limbs in the anterior horn. Repaired limbs had prolonged cartilage T2 values, primarily anteriorly, and tended to have lower biomechanical force to failure at 8 months than Non-Op limbs. MPM autofluorescence and second harmonic generation data correlated with T2* values at 8 months (ρ=-0.48, P=0.06). Conclusions: T2*mapping is sensitive to detecting temporal and zonal differences of meniscal structure and composition. Meniscal MPM and cartilage T2 values indicate changes in tissue integrity in the presence of meniscal repair.

Original language	English
Pages (from-to)	1083-1091
Number of pages	9
Journal	Osteoarthritis and Cartilage
Volume	21
Issue number	8
DOIs	https://doi.org/10.1016/j.joca.2013.04.020
Publication status	Published - Aug 2013

Bibliographical note

Funding Information:
Research reported in this publication was supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health under Award Number RC1-AR058255. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Funding

Research reported in this publication was supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health under Award Number RC1-AR058255. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Funders	Funder number
National Institutes of Health
National Institute of Arthritis and Musculoskeletal and Skin Diseases	RC1AR058255

ASJC Scopus Subject Areas

Rheumatology
Biomedical Engineering
Orthopedics and Sports Medicine

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10.1016/j.joca.2013.04.020

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title = "Correlation of meniscal T2* with multiphoton microscopy, and change of articular cartilage T2 in an ovine model of meniscal repair",

abstract = "Objective: To correlate meniscal T2* relaxation times using ultra-short echo time (UTE) magnetic resonance imaging (MRI) with quantitative microscopic methods, and to determine the effect of meniscal repair on post-operative cartilage T2 values. Design: A medial meniscal tear was created and repaired in the anterior horn of one limb of 28 crossbred mature ewes. MR scans for morphological evaluation, meniscal T2* values, and cartilage T2 values were acquired at 0, 4 and 8 months post-operatively for the Tear and Non-Op limb. Samples of menisci from both limbs were analyzed using multiphoton microscopy (MPM) analysis and biomechanical testing. Results: Significantly prolonged meniscal T2* values were found in repaired limbs than in control limbs, P<0.0001. No regional differences of T2* were detected for either the repaired or control limbs in the anterior horn. Repaired limbs had prolonged cartilage T2 values, primarily anteriorly, and tended to have lower biomechanical force to failure at 8 months than Non-Op limbs. MPM autofluorescence and second harmonic generation data correlated with T2* values at 8 months (ρ=-0.48, P=0.06). Conclusions: T2*mapping is sensitive to detecting temporal and zonal differences of meniscal structure and composition. Meniscal MPM and cartilage T2 values indicate changes in tissue integrity in the presence of meniscal repair.",

author = "Koff, {M. F.} and P. Shah and S. Pownder and B. Romero and R. Williams and S. Gilbert and S. Maher and Fortier, {L. A.} and Rodeo, {S. A.} and Potter, {H. G.}",

note = "Funding Information: Research reported in this publication was supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health under Award Number RC1-AR058255. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. ",

year = "2013",

month = aug,

doi = "10.1016/j.joca.2013.04.020",

language = "English",

volume = "21",

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journal = "Osteoarthritis and Cartilage",

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T1 - Correlation of meniscal T2* with multiphoton microscopy, and change of articular cartilage T2 in an ovine model of meniscal repair

AU - Koff, M. F.

AU - Shah, P.

AU - Pownder, S.

AU - Romero, B.

AU - Williams, R.

AU - Gilbert, S.

AU - Maher, S.

AU - Fortier, L. A.

AU - Rodeo, S. A.

AU - Potter, H. G.

N1 - Funding Information: Research reported in this publication was supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health under Award Number RC1-AR058255. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

PY - 2013/8

Y1 - 2013/8

N2 - Objective: To correlate meniscal T2* relaxation times using ultra-short echo time (UTE) magnetic resonance imaging (MRI) with quantitative microscopic methods, and to determine the effect of meniscal repair on post-operative cartilage T2 values. Design: A medial meniscal tear was created and repaired in the anterior horn of one limb of 28 crossbred mature ewes. MR scans for morphological evaluation, meniscal T2* values, and cartilage T2 values were acquired at 0, 4 and 8 months post-operatively for the Tear and Non-Op limb. Samples of menisci from both limbs were analyzed using multiphoton microscopy (MPM) analysis and biomechanical testing. Results: Significantly prolonged meniscal T2* values were found in repaired limbs than in control limbs, P<0.0001. No regional differences of T2* were detected for either the repaired or control limbs in the anterior horn. Repaired limbs had prolonged cartilage T2 values, primarily anteriorly, and tended to have lower biomechanical force to failure at 8 months than Non-Op limbs. MPM autofluorescence and second harmonic generation data correlated with T2* values at 8 months (ρ=-0.48, P=0.06). Conclusions: T2*mapping is sensitive to detecting temporal and zonal differences of meniscal structure and composition. Meniscal MPM and cartilage T2 values indicate changes in tissue integrity in the presence of meniscal repair.

AB - Objective: To correlate meniscal T2* relaxation times using ultra-short echo time (UTE) magnetic resonance imaging (MRI) with quantitative microscopic methods, and to determine the effect of meniscal repair on post-operative cartilage T2 values. Design: A medial meniscal tear was created and repaired in the anterior horn of one limb of 28 crossbred mature ewes. MR scans for morphological evaluation, meniscal T2* values, and cartilage T2 values were acquired at 0, 4 and 8 months post-operatively for the Tear and Non-Op limb. Samples of menisci from both limbs were analyzed using multiphoton microscopy (MPM) analysis and biomechanical testing. Results: Significantly prolonged meniscal T2* values were found in repaired limbs than in control limbs, P<0.0001. No regional differences of T2* were detected for either the repaired or control limbs in the anterior horn. Repaired limbs had prolonged cartilage T2 values, primarily anteriorly, and tended to have lower biomechanical force to failure at 8 months than Non-Op limbs. MPM autofluorescence and second harmonic generation data correlated with T2* values at 8 months (ρ=-0.48, P=0.06). Conclusions: T2*mapping is sensitive to detecting temporal and zonal differences of meniscal structure and composition. Meniscal MPM and cartilage T2 values indicate changes in tissue integrity in the presence of meniscal repair.

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Correlation of meniscal T2* with multiphoton microscopy, and change of articular cartilage T2 in an ovine model of meniscal repair

Abstract

Bibliographical note

Funding

ASJC Scopus Subject Areas

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