Design, synthesis and cellular dynamics studies in membranes of a new coumarin-based "turn-off" fluorescent probe selective for Fe 2+

Olimpo García-Beltrán; Natalia Mena; Osvaldo Yañez; Julio Caballero; Víctor Vargas; Marco T. Nuñez; Bruce K. Cassels

doi:10.1016/j.ejmech.2013.06.022

Design, synthesis and cellular dynamics studies in membranes of a new coumarin-based "turn-off" fluorescent probe selective for Fe ²⁺

Olimpo García-Beltrán, Natalia Mena, Osvaldo Yañez, Julio Caballero, Víctor Vargas, Marco T. Nuñez, Bruce K. Cassels

Olimpo

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37 Citations (Scopus)

Abstract

A new coumarin-based 'turn-off' fluorescent probe, 7-(diethylamino)-N-(1,3- dihydroxy-2-(hydroxymethyl) propan-2-yl)-2-oxo-2H-chromene-3-carboxamide (AGD) was synthesized. This compound is highly selective for ferrous ions (Fe ²⁺) and can reversibly detect them in aqueous medium. The probe localizes to the cell membrane in living cells, where it can detect changes in Fe²⁺ concentration. Molecular dynamics (MD) simulations indicate that AGD interacts with the lipid bilayer at the level of the glycerol moieties.

Original language	English
Pages (from-to)	60-63
Number of pages	4
Journal	European Journal of Medicinal Chemistry
Volume	67
DOIs	https://doi.org/10.1016/j.ejmech.2013.06.022
Publication status	Published - 2013

ASJC Scopus Subject Areas

Pharmacology
Drug Discovery
Organic Chemistry

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García-Beltrán, O., Mena, N., Yañez, O., Caballero, J., Vargas, V., Nuñez, M. T., & Cassels, B. K. (2013). Design, synthesis and cellular dynamics studies in membranes of a new coumarin-based "turn-off" fluorescent probe selective for Fe ²⁺. European Journal of Medicinal Chemistry, 67, 60-63. https://doi.org/10.1016/j.ejmech.2013.06.022

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AU - García-Beltrán, Olimpo

AU - Mena, Natalia

AU - Yañez, Osvaldo

AU - Caballero, Julio

AU - Vargas, Víctor

AU - Nuñez, Marco T.

AU - Cassels, Bruce K.

PY - 2013

Y1 - 2013

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Design, synthesis and cellular dynamics studies in membranes of a new coumarin-based "turn-off" fluorescent probe selective for Fe ²⁺

Abstract

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