Direct measurement of apoprotein B specific activity in 125I-labeled lipoproteins

N. A. Le; J. S. Melish; B. C. Roach; H. N. Ginsberg; W. V. Brown

Direct measurement of apoprotein B specific activity in ¹²⁵I-labeled lipoproteins

N. A. Le, J. S. Melish, B. C. Roach, H. N. Ginsberg, W. V. Brown

Vagelos College of Physicians and Surgeons

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

A method for determining apoprotein B specific activity in radioiodinated lipoproteins is described and validated. It utilizes organic solvents and tetramethylurea in the isolation of apoprotein B from other radiolabeled contaminants, both lipid and protein, in exogenously labeled VLDL. The contaminants are also removed from those lipoprotein classes subsequently derived from VLDL, namely IDL and LDL. The procedure requires approximately 50 μg of apoB per analysis, allowing specific activity determinations in triplicate on 3-ml plasma samples with a standard error of <6%. Finally, data from a study of apoprotein B turnover in VLDL, IDL, and LDL in a human subject is presented to demonstrate the potential of this method in further elucidating the kinetic interrelationships between these lipoprotein classes.

Original language	English
Pages (from-to)	578-584
Number of pages	7
Journal	Unknown Journal
Volume	19
Issue number	5
Publication status	Published - 1978

ASJC Scopus Subject Areas

Biochemistry
Endocrinology
Cell Biology

Cite this

Le, N. A., Melish, J. S., Roach, B. C., Ginsberg, H. N., & Brown, W. V. (1978). Direct measurement of apoprotein B specific activity in ¹²⁵I-labeled lipoproteins. Unknown Journal, 19(5), 578-584.

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abstract = "A method for determining apoprotein B specific activity in radioiodinated lipoproteins is described and validated. It utilizes organic solvents and tetramethylurea in the isolation of apoprotein B from other radiolabeled contaminants, both lipid and protein, in exogenously labeled VLDL. The contaminants are also removed from those lipoprotein classes subsequently derived from VLDL, namely IDL and LDL. The procedure requires approximately 50 μg of apoB per analysis, allowing specific activity determinations in triplicate on 3-ml plasma samples with a standard error of <6%. Finally, data from a study of apoprotein B turnover in VLDL, IDL, and LDL in a human subject is presented to demonstrate the potential of this method in further elucidating the kinetic interrelationships between these lipoprotein classes.",

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AU - Le, N. A.

AU - Melish, J. S.

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AU - Brown, W. V.

PY - 1978

Y1 - 1978

N2 - A method for determining apoprotein B specific activity in radioiodinated lipoproteins is described and validated. It utilizes organic solvents and tetramethylurea in the isolation of apoprotein B from other radiolabeled contaminants, both lipid and protein, in exogenously labeled VLDL. The contaminants are also removed from those lipoprotein classes subsequently derived from VLDL, namely IDL and LDL. The procedure requires approximately 50 μg of apoB per analysis, allowing specific activity determinations in triplicate on 3-ml plasma samples with a standard error of <6%. Finally, data from a study of apoprotein B turnover in VLDL, IDL, and LDL in a human subject is presented to demonstrate the potential of this method in further elucidating the kinetic interrelationships between these lipoprotein classes.

AB - A method for determining apoprotein B specific activity in radioiodinated lipoproteins is described and validated. It utilizes organic solvents and tetramethylurea in the isolation of apoprotein B from other radiolabeled contaminants, both lipid and protein, in exogenously labeled VLDL. The contaminants are also removed from those lipoprotein classes subsequently derived from VLDL, namely IDL and LDL. The procedure requires approximately 50 μg of apoB per analysis, allowing specific activity determinations in triplicate on 3-ml plasma samples with a standard error of <6%. Finally, data from a study of apoprotein B turnover in VLDL, IDL, and LDL in a human subject is presented to demonstrate the potential of this method in further elucidating the kinetic interrelationships between these lipoprotein classes.

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Direct measurement of apoprotein B specific activity in ¹²⁵I-labeled lipoproteins

Abstract

ASJC Scopus Subject Areas

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