Genome-wide analysis of periodontal and peri-implant cells and tissues

Moritz Kebschull; Claudia Hülsmann; Per Hoffmann; Panos N. Papapanou

doi:10.1007/978-1-4939-6685-1_18

Genome-wide analysis of periodontal and peri-implant cells and tissues

Moritz Kebschull, Claudia Hülsmann, Per Hoffmann, Panos N. Papapanou

College of Dental Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter

6 Citations (Scopus)

Abstract

Omics analyses, including the systematic cataloging of messenger RNA and microRNA sequences or DNA methylation patterns in a cell population, organ or tissue sample, are powerful means of generating comprehensive genome-level data sets on complex diseases. We have systematically assessed the transcriptome, miRNome and methylome of gingival tissues from subjects with different diagnostic entities of periodontal disease, and studied the transcriptome of primary cells ex vivo, or in vitro after infection with periodontal pathogens. Our data further our understanding of the pathobiology of periodontal diseases and indicate that the gingival -omes translate into discernible phenotypic characteristics and possibly support an alternative, “molecular” classification of periodontitis. Here, we outline the laboratory steps required for the processing of periodontal cells and tissues foromics analyses using current microarrays or next-generation sequencing technology.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	307-326
Number of pages	20
DOIs	https://doi.org/10.1007/978-1-4939-6685-1_18
Publication status	Published - 2017

Publication series

Name	Methods in Molecular Biology
Volume	1537
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media LLC 2017.

Funding

This work was supported by grants from the German Society for Periodontology (DG PARO) and the German Society for Oral and Maxillofacial Sciences (DGZMK) to M.K. and by grants from NIH/NIDCR (DE015649, DE021820, and DE024735) and by an unrestricted gift from Colgate-Palmolive Inc. to P.N.P.

Funders	Funder number
Colgate-Palmolive Inc.
German Society for Periodontology
NIH/NIDCR	DE021820, DE024735, DE015649
National Institute of Dental and Craniofacial Research	R21DE021820

ASJC Scopus Subject Areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-6685-1_18

Cite this

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abstract = "Omics analyses, including the systematic cataloging of messenger RNA and microRNA sequences or DNA methylation patterns in a cell population, organ or tissue sample, are powerful means of generating comprehensive genome-level data sets on complex diseases. We have systematically assessed the transcriptome, miRNome and methylome of gingival tissues from subjects with different diagnostic entities of periodontal disease, and studied the transcriptome of primary cells ex vivo, or in vitro after infection with periodontal pathogens. Our data further our understanding of the pathobiology of periodontal diseases and indicate that the gingival -omes translate into discernible phenotypic characteristics and possibly support an alternative, “molecular” classification of periodontitis. Here, we outline the laboratory steps required for the processing of periodontal cells and tissues foromics analyses using current microarrays or next-generation sequencing technology.",

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Genome-wide analysis of periodontal and peri-implant cells and tissues

Abstract

Publication series

Bibliographical note

Funding

ASJC Scopus Subject Areas

Access to Document

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