Inhibition of mixed leukocyte culture reactivity by sera from hemodialysis patients and transplant recipients as an indicator of isoimmunization

N. Suciu-Foca; J. A. Buda; T. Thiem; P. Almojera; K. B. Reemtsma

Inhibition of mixed leukocyte culture reactivity by sera from hemodialysis patients and transplant recipients as an indicator of isoimmunization

N. Suciu-Foca, J. A. Buda, T. Thiem, P. Almojera, K. B. Reemtsma

Vagelos College of Physicians and Surgeons

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A system has been designed to determine the mixed leukocyte culture (MLC) inhibitory activity of sera from patients presensitized by hemodialysis or transplantation. Monthly samples of sera were screened for blocking factors on 10 different one way MLC, using a constant panel of responding lymphocytes and a standard stimulating cell mixture. In addition, the reactivity of the patients' lymphocytes to the same stimulating cell mixture was tested. MLC inhibitory activity was detectable before appearance or following the disappearance of lymphocytotoxic antibodies in sera from hemodialysis patients. In transplant recipients MLC inhibitory activity was strongly associated with cytotoxic antibodies and humoral rejection. These data suggest the value of MLC inhibition as a sensitive test for detection of presensitization.

Original language	English
Pages (from-to)	1-5
Number of pages	5
Journal	Laboratory Investigation
Volume	31
Issue number	1
Publication status	Published - 1974

ASJC Scopus Subject Areas

General Medicine

Cite this

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AU - Suciu-Foca, N.

AU - Buda, J. A.

AU - Thiem, T.

AU - Almojera, P.

AU - Reemtsma, K. B.

PY - 1974

Y1 - 1974

N2 - A system has been designed to determine the mixed leukocyte culture (MLC) inhibitory activity of sera from patients presensitized by hemodialysis or transplantation. Monthly samples of sera were screened for blocking factors on 10 different one way MLC, using a constant panel of responding lymphocytes and a standard stimulating cell mixture. In addition, the reactivity of the patients' lymphocytes to the same stimulating cell mixture was tested. MLC inhibitory activity was detectable before appearance or following the disappearance of lymphocytotoxic antibodies in sera from hemodialysis patients. In transplant recipients MLC inhibitory activity was strongly associated with cytotoxic antibodies and humoral rejection. These data suggest the value of MLC inhibition as a sensitive test for detection of presensitization.

AB - A system has been designed to determine the mixed leukocyte culture (MLC) inhibitory activity of sera from patients presensitized by hemodialysis or transplantation. Monthly samples of sera were screened for blocking factors on 10 different one way MLC, using a constant panel of responding lymphocytes and a standard stimulating cell mixture. In addition, the reactivity of the patients' lymphocytes to the same stimulating cell mixture was tested. MLC inhibitory activity was detectable before appearance or following the disappearance of lymphocytotoxic antibodies in sera from hemodialysis patients. In transplant recipients MLC inhibitory activity was strongly associated with cytotoxic antibodies and humoral rejection. These data suggest the value of MLC inhibition as a sensitive test for detection of presensitization.

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Inhibition of mixed leukocyte culture reactivity by sera from hemodialysis patients and transplant recipients as an indicator of isoimmunization

Abstract

ASJC Scopus Subject Areas

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