Introduction and expression of a rabbit beta-globin gene in mouse fibroblasts.

B. Wold; M. Wigler; E. Lacy; T. Maniatis; S. Silverstein; R. Axel

doi:10.1073/pnas.76.11.5684

Introduction and expression of a rabbit beta-globin gene in mouse fibroblasts.

B. Wold, M. Wigler, E. Lacy, T. Maniatis, S. Silverstein, R. Axel

Zuckerman Institute

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Abstract

The cloned chromosomal rabbit beta-globin gene has been introduced into mouse fibroblasts by DNA-mediated gene transfer (transformation). In this report, we examine the expression of the rabbit gene in six independent transformants that contain from 1 to 20 copies of the cloned globin gene. Rabbit globin transcripts were detected in two of these transformants at steady-state concentrations of 5 and 2 copies per cell. The globin transcripts from one cell line are polyadenylylated and migrate as 9S RNA on methylmercury gels. These transcripts reflect correct processing of the two intervening sequences but lack 48 +/- 5 nucleotides present at the 5' terminus of rabbit erythrocyte globin mRNA.

Original language	English
Pages (from-to)	5684-5688
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	76
Issue number	11
DOIs	https://doi.org/10.1073/pnas.76.11.5684
Publication status	Published - Nov 1979

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abstract = "The cloned chromosomal rabbit beta-globin gene has been introduced into mouse fibroblasts by DNA-mediated gene transfer (transformation). In this report, we examine the expression of the rabbit gene in six independent transformants that contain from 1 to 20 copies of the cloned globin gene. Rabbit globin transcripts were detected in two of these transformants at steady-state concentrations of 5 and 2 copies per cell. The globin transcripts from one cell line are polyadenylylated and migrate as 9S RNA on methylmercury gels. These transcripts reflect correct processing of the two intervening sequences but lack 48 +/- 5 nucleotides present at the 5' terminus of rabbit erythrocyte globin mRNA.",

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AU - Wold, B.

AU - Wigler, M.

AU - Lacy, E.

AU - Maniatis, T.

AU - Silverstein, S.

AU - Axel, R.

PY - 1979/11

Y1 - 1979/11

N2 - The cloned chromosomal rabbit beta-globin gene has been introduced into mouse fibroblasts by DNA-mediated gene transfer (transformation). In this report, we examine the expression of the rabbit gene in six independent transformants that contain from 1 to 20 copies of the cloned globin gene. Rabbit globin transcripts were detected in two of these transformants at steady-state concentrations of 5 and 2 copies per cell. The globin transcripts from one cell line are polyadenylylated and migrate as 9S RNA on methylmercury gels. These transcripts reflect correct processing of the two intervening sequences but lack 48 +/- 5 nucleotides present at the 5' terminus of rabbit erythrocyte globin mRNA.

AB - The cloned chromosomal rabbit beta-globin gene has been introduced into mouse fibroblasts by DNA-mediated gene transfer (transformation). In this report, we examine the expression of the rabbit gene in six independent transformants that contain from 1 to 20 copies of the cloned globin gene. Rabbit globin transcripts were detected in two of these transformants at steady-state concentrations of 5 and 2 copies per cell. The globin transcripts from one cell line are polyadenylylated and migrate as 9S RNA on methylmercury gels. These transcripts reflect correct processing of the two intervening sequences but lack 48 +/- 5 nucleotides present at the 5' terminus of rabbit erythrocyte globin mRNA.

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Introduction and expression of a rabbit beta-globin gene in mouse fibroblasts.

Abstract

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