Modifications of Ribonucleic Acid by Chemical Carcinogens. I. in Vitro Modification of Transfer Ribonucleic Acid by N-Acetoxy-2-acetylaminofluorene

Louis M. Fink; Susumu Nishimura; I. Bernard Weinstein

doi:10.1021/bi00805a007

Modifications of Ribonucleic Acid by Chemical Carcinogens. I. in Vitro Modification of Transfer Ribonucleic Acid by N-Acetoxy-2-acetylaminofluorene

Louis M. Fink, Susumu Nishimura, I. Bernard Weinstein

Vagelos College of Physicians and Surgeons

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

Escherichii coli transfer ribonucleic acid was reacted in vitro with the carcinogen N-acetoxy-2-acetylaminofluorene. Evidence is presented that covalent attachment of the acetylaminofluorene residues to transfer ribonucleic acids produces specific modifications in their amino acid acceptance capacities, codon recognition and ribosomal binding, and chromatographic behavior on DEAE-Sephadex and benzoylated DEAE-cellulose columns. An hypothesis is presented that attributes the functional modifications in nucleic acids produced by this carcinogen to a change in nucleoside conformation from anti to syn.

Original language	English
Pages (from-to)	496-502
Number of pages	7
Journal	Biochemistry
Volume	9
Issue number	3
DOIs	https://doi.org/10.1021/bi00805a007
Publication status	Published - Feb 1 1970

ASJC Scopus Subject Areas

Biochemistry

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abstract = "Escherichii coli transfer ribonucleic acid was reacted in vitro with the carcinogen N-acetoxy-2-acetylaminofluorene. Evidence is presented that covalent attachment of the acetylaminofluorene residues to transfer ribonucleic acids produces specific modifications in their amino acid acceptance capacities, codon recognition and ribosomal binding, and chromatographic behavior on DEAE-Sephadex and benzoylated DEAE-cellulose columns. An hypothesis is presented that attributes the functional modifications in nucleic acids produced by this carcinogen to a change in nucleoside conformation from anti to syn.",

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N2 - Escherichii coli transfer ribonucleic acid was reacted in vitro with the carcinogen N-acetoxy-2-acetylaminofluorene. Evidence is presented that covalent attachment of the acetylaminofluorene residues to transfer ribonucleic acids produces specific modifications in their amino acid acceptance capacities, codon recognition and ribosomal binding, and chromatographic behavior on DEAE-Sephadex and benzoylated DEAE-cellulose columns. An hypothesis is presented that attributes the functional modifications in nucleic acids produced by this carcinogen to a change in nucleoside conformation from anti to syn.

AB - Escherichii coli transfer ribonucleic acid was reacted in vitro with the carcinogen N-acetoxy-2-acetylaminofluorene. Evidence is presented that covalent attachment of the acetylaminofluorene residues to transfer ribonucleic acids produces specific modifications in their amino acid acceptance capacities, codon recognition and ribosomal binding, and chromatographic behavior on DEAE-Sephadex and benzoylated DEAE-cellulose columns. An hypothesis is presented that attributes the functional modifications in nucleic acids produced by this carcinogen to a change in nucleoside conformation from anti to syn.

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Modifications of Ribonucleic Acid by Chemical Carcinogens. I. in Vitro Modification of Transfer Ribonucleic Acid by N-Acetoxy-2-acetylaminofluorene

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