TY - JOUR
T1 - Porphyromonas gingivalis infection and cell death in human aortic endothelial cells
AU - Roth, Georg A.
AU - Ankersmit, Hendrik J.
AU - Brown, Vinette B.
AU - Papapanou, Panos N.
AU - Schmidt, Ann Marie
AU - Lalla, Evanthia
PY - 2007/7
Y1 - 2007/7
N2 - Porphyromonas gingivalis is a periodontal pathogen that promotes a proatherogenic response in endothelial cells. Cell death responses of human aortic endothelial cells to P. gingivalis at various multiplicities of infection (MOI) were investigated by assessment of cell detachment, histone-associated DNA fragmentation, lactate dehydrogenase release and ADP:ATP ratio. Porphyromonas gingivalis at MOI 1 : 10-1 : 100 did not have a cytotoxic effect, but induced apoptotic cell death at MOI 1 : 500 and 1 : 1000. Monocyte chemoattractant protein-1 production was significantly enhanced by P. gingivalis at MOI 1 : 100. At higher MOI, at least in vitro, P. gingivalis mediates endothelial apoptosis, thereby potentially amplifying proatherogenic mechanisms in the perturbed vasculature.
AB - Porphyromonas gingivalis is a periodontal pathogen that promotes a proatherogenic response in endothelial cells. Cell death responses of human aortic endothelial cells to P. gingivalis at various multiplicities of infection (MOI) were investigated by assessment of cell detachment, histone-associated DNA fragmentation, lactate dehydrogenase release and ADP:ATP ratio. Porphyromonas gingivalis at MOI 1 : 10-1 : 100 did not have a cytotoxic effect, but induced apoptotic cell death at MOI 1 : 500 and 1 : 1000. Monocyte chemoattractant protein-1 production was significantly enhanced by P. gingivalis at MOI 1 : 100. At higher MOI, at least in vitro, P. gingivalis mediates endothelial apoptosis, thereby potentially amplifying proatherogenic mechanisms in the perturbed vasculature.
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U2 - 10.1111/j.1574-6968.2007.00736.x
DO - 10.1111/j.1574-6968.2007.00736.x
M3 - Article
C2 - 17459112
AN - SCOPUS:34250627288
SN - 0378-1097
VL - 272
SP - 106
EP - 113
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1
ER -