Extensive multiplexing of protein nucleic-acid interactions to comprehensively study gene expression regulation from chromatin to mRNA degradation

Public Health Relevancy Statement Gene expression is tightly controlled in time and space and is governed by protein-nucleic acid (DNA and RNA) interactions. There are hundreds of DNA- and RNA-binding proteins that regulate gene expression and many are mutated in disease, but we lack facile approaches to map these proteins to their DNA/RNA targets. Here we propose to develop an innovative set of tools that will give every molecular biology lab the possibility to obtain comprehensive cell-type specific protein DNA/RNA interaction maps, a necessity to understand gene expression regulation and therefore disease development.