A DNA-modification methylase from Bacillus stearothermophilus V

R. Barra; M. Chiong; E. Gonzalez; C. Vasquez

A DNA-modification methylase from Bacillus stearothermophilus V

R. Barra, M. Chiong, E. Gonzalez, C. Vasquez

Universidad de Chile

Producción científica › revisión exhaustiva

13 Citas (Scopus)

Resumen

A type II modification methylase (M BstVI) was partially purified from the thermophilic bacterium Bacillus stearothermophilus V. The methylase catalyses the transfer of methyl groups from S-adenosyl-L-methionine to unmodified double-stranded DNA. The product of methylation was identified by paper chromatography as N⁶-methyladenine. Since M BstVI protects DNA against cleavage by BstVI and XhoI restriction endonucleases, it follows that it methylates the adenine residue in the sequence 5'-C-T-C-G-A-G-3'.

Idioma original	English
Páginas (desde-hasta)	699-703
Número de páginas	5
Publicación	Biochemical Journal
Volumen	255
N.º	2
Estado	Published - 1988

ASJC Scopus Subject Areas

Biochemistry
Molecular Biology
Cell Biology

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Barra, R., Chiong, M., Gonzalez, E., & Vasquez, C. (1988). A DNA-modification methylase from Bacillus stearothermophilus V. Biochemical Journal, 255(2), 699-703.

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AU - Chiong, M.

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AU - Vasquez, C.

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N2 - A type II modification methylase (M BstVI) was partially purified from the thermophilic bacterium Bacillus stearothermophilus V. The methylase catalyses the transfer of methyl groups from S-adenosyl-L-methionine to unmodified double-stranded DNA. The product of methylation was identified by paper chromatography as N6-methyladenine. Since M BstVI protects DNA against cleavage by BstVI and XhoI restriction endonucleases, it follows that it methylates the adenine residue in the sequence 5'-C-T-C-G-A-G-3'.

AB - A type II modification methylase (M BstVI) was partially purified from the thermophilic bacterium Bacillus stearothermophilus V. The methylase catalyses the transfer of methyl groups from S-adenosyl-L-methionine to unmodified double-stranded DNA. The product of methylation was identified by paper chromatography as N6-methyladenine. Since M BstVI protects DNA against cleavage by BstVI and XhoI restriction endonucleases, it follows that it methylates the adenine residue in the sequence 5'-C-T-C-G-A-G-3'.

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A DNA-modification methylase from Bacillus stearothermophilus V

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