Effect of inhibitors of signal transduction on IGF-1-induced protein synthesis associated with hypertrophy in cultured neonatal rat ventricular myocytes

Sergio Lavandero; Rocío Foncea; Viviana Pérez; Mario Sapag-Hagar

doi:10.1016/S0014-5793(98)00008-8

Effect of inhibitors of signal transduction on IGF-1-induced protein synthesis associated with hypertrophy in cultured neonatal rat ventricular myocytes

Sergio Lavandero, Rocío Foncea, Viviana Pérez, Mario Sapag-Hagar

Universidad de Chile

Producción científica › revisión exhaustiva

48 Citas (Scopus)

Resumen

IGF-1 increased 2-fold protein synthesis in cardiac myocytes. Genistein, whether added during preincubation or with IGF-1 at the start of incubation, significantly inhibited the IGF-1-induced stimulation of protein synthesis, autophosphorylation of the β-subunit of IGF-1 receptor and inhibition of ERK. When added 1 or 6 h after IGF-1, however, genistein was without effect. IGF-1-stimulated protein synthesis was also significantly inhibited by PD-098059, staurosporine, and rapamycin, but not by wortmannin, in cardiac myocytes. Some inhibitors produced a reduction in cell size. Activation of the ERK cascade by IGF-1 may be responsible for some of the features associated with cardiac myocyte hypertrophy.

Idioma original	English
Páginas (desde-hasta)	193-196
Número de páginas	4
Publicación	FEBS Letters
Volumen	422
N.º	2
DOI	https://doi.org/10.1016/S0014-5793(98)00008-8
Estado	Published - ene. 30 1998

Financiación

We thank Dr. C.I. Pogson for help with the preparation of the text of this paper. This work was supported in part by Fondo Nacional de Ciencia y Tecnologı́a (FONDECYT) Grants 1950452 (to S.L.) and 2950002 (to R.F.). R.F. is the recipient of a CONICYT fellowship (Chile).

Financiadores	Número del financiador
Comisión Nacional de Investigación Científica y Tecnológica
Fondo Nacional de Ciencia y Tecnología	1950452, 2950002

ASJC Scopus Subject Areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Acceder al documento

10.1016/S0014-5793(98)00008-8

Otros archivos y enlaces

Citar esto

@article{2da73a13a965497eb5af8287f7f538d7,

title = "Effect of inhibitors of signal transduction on IGF-1-induced protein synthesis associated with hypertrophy in cultured neonatal rat ventricular myocytes",

abstract = "IGF-1 increased 2-fold protein synthesis in cardiac myocytes. Genistein, whether added during preincubation or with IGF-1 at the start of incubation, significantly inhibited the IGF-1-induced stimulation of protein synthesis, autophosphorylation of the β-subunit of IGF-1 receptor and inhibition of ERK. When added 1 or 6 h after IGF-1, however, genistein was without effect. IGF-1-stimulated protein synthesis was also significantly inhibited by PD-098059, staurosporine, and rapamycin, but not by wortmannin, in cardiac myocytes. Some inhibitors produced a reduction in cell size. Activation of the ERK cascade by IGF-1 may be responsible for some of the features associated with cardiac myocyte hypertrophy.",

author = "Sergio Lavandero and Roc{\'i}o Foncea and Viviana P{\'e}rez and Mario Sapag-Hagar",

note = "Funding Information: We thank Dr. C.I. Pogson for help with the preparation of the text of this paper. This work was supported in part by Fondo Nacional de Ciencia y Tecnolog{\'ı}a (FONDECYT) Grants 1950452 (to S.L.) and 2950002 (to R.F.). R.F. is the recipient of a CONICYT fellowship (Chile).",

year = "1998",

month = jan,

day = "30",

doi = "10.1016/S0014-5793(98)00008-8",

language = "English",

volume = "422",

pages = "193--196",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Effect of inhibitors of signal transduction on IGF-1-induced protein synthesis associated with hypertrophy in cultured neonatal rat ventricular myocytes

AU - Lavandero, Sergio

AU - Foncea, Rocío

AU - Pérez, Viviana

AU - Sapag-Hagar, Mario

N1 - Funding Information: We thank Dr. C.I. Pogson for help with the preparation of the text of this paper. This work was supported in part by Fondo Nacional de Ciencia y Tecnologı́a (FONDECYT) Grants 1950452 (to S.L.) and 2950002 (to R.F.). R.F. is the recipient of a CONICYT fellowship (Chile).

PY - 1998/1/30

Y1 - 1998/1/30

N2 - IGF-1 increased 2-fold protein synthesis in cardiac myocytes. Genistein, whether added during preincubation or with IGF-1 at the start of incubation, significantly inhibited the IGF-1-induced stimulation of protein synthesis, autophosphorylation of the β-subunit of IGF-1 receptor and inhibition of ERK. When added 1 or 6 h after IGF-1, however, genistein was without effect. IGF-1-stimulated protein synthesis was also significantly inhibited by PD-098059, staurosporine, and rapamycin, but not by wortmannin, in cardiac myocytes. Some inhibitors produced a reduction in cell size. Activation of the ERK cascade by IGF-1 may be responsible for some of the features associated with cardiac myocyte hypertrophy.

AB - IGF-1 increased 2-fold protein synthesis in cardiac myocytes. Genistein, whether added during preincubation or with IGF-1 at the start of incubation, significantly inhibited the IGF-1-induced stimulation of protein synthesis, autophosphorylation of the β-subunit of IGF-1 receptor and inhibition of ERK. When added 1 or 6 h after IGF-1, however, genistein was without effect. IGF-1-stimulated protein synthesis was also significantly inhibited by PD-098059, staurosporine, and rapamycin, but not by wortmannin, in cardiac myocytes. Some inhibitors produced a reduction in cell size. Activation of the ERK cascade by IGF-1 may be responsible for some of the features associated with cardiac myocyte hypertrophy.

UR - http://www.scopus.com/inward/record.url?scp=0032579211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032579211&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(98)00008-8

DO - 10.1016/S0014-5793(98)00008-8

M3 - Article

C2 - 9490004

AN - SCOPUS:0032579211

SN - 0014-5793

VL - 422

SP - 193

EP - 196

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Effect of inhibitors of signal transduction on IGF-1-induced protein synthesis associated with hypertrophy in cultured neonatal rat ventricular myocytes

Resumen

Financiación

ASJC Scopus Subject Areas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto