Détails sur le projet
Description
Trabecular bone adaptation plays a significant role in the etiology of
many metabolic bone diseases such as osteoporosis, osteopetrosis, bone
loss in microgravity and the long term success or failure of porous
implants in total joint arthroplasty. Parathyroid hormone (PTH) is an
important anabolic agent when administrated intermittently. The long-
term goal of this research program is to use an in vivo model to study
the biophysical signal transduction pathways mediating bone remodeling.
In this proposed project, the trabecular bone response to combined
mechanical loading and PTH stimulation will be quantified utilizing a
novel in vivo rat tail vertebra model coupled with micro-CT image based
finite element technique. Specifically, a controlled mechanical load
will be applied to a rat tail vertebra and detailed 3D stress/strain
environment in the trabecular bone tissue will be determined using an
advanced finite element microstructural model of the same vertebra. The
cellular response will be quantified using histomorphometric techniques
in the presence or absence of intermittent PTH treatment. This approach
will help to elucidate the separate and combined effects of mechanical
and hormonal stimulations on bone cell response in vivo.
The specific aims of this projects are:
Specific Aim 1: To determine the in vivo cellular responses and 3D bone
morphology to intermittent PTH treatment in rat tail vertebrae and their
relationships to duration of treatment (2,4, and 8 weeks).
Specific Aim 2: To determine the in vivo cellular responses and 3D bone
morphology to mechanical stimulation in rat tail vertebrae and their
relationship to loading levels (0 N:Immobilized, 100 N:Load and Sham),
bone tissue stress/strain environment and duration of treatment (2,4,
and 8 weeks).
Specific Aim 3: To determine the in vivo cellular responses and 3D bone
morphology to combined PTH treatment and mechanical stimulation in rat
tail vertebrae and their relationships to sequence of stimulation (PTH-
Load; Load-PTH; PTH-Immobilized; Immobilized-PTH; PTH-Sham; Sham-PTH),
bone tissue stress/strain environment and duration of treatment (2,4,
8 weeks).
Results from this pilot study may have significant implications with
respect to the roles of mechanical forces and PTH in bone mass
homeostasis.
Statut | Terminé |
---|---|
Date de début/de fin réelle | 9/28/98 → 8/31/01 |
Financement
- National Institute of Arthritis and Musculoskeletal and Skin Diseases: 83 170,00 $ US
Keywords
- Radiología, medicina nuclear y obtención de imágenes
- Reumatología
Empreinte numérique
Explorer les sujets de recherche abordés dans ce projet. Ces étiquettes sont créées en fonction des prix/bourses sous-jacents. Ensemble, ils forment une empreinte numérique unique.