Détails sur le projet
Description
This is a competing renewal application for a grant investigating
the role of Na-coupled anion transport processes in the absorption
and secretion of electrolytes by intestine and other epithelia
(kidney, trachea). Two cotransport systems will be studied: The
first is the Na-K-Cl cotransproter, the functions and regulation
of which were well described in our prior 2 grant periods. We
propose now to continue studies already begun on the purification
of the cotransport protein, to reconstitute the cotransporter by
adding solubilized membrane proteins to liposomes and to clone the
cDNA for the cotransporter using the Xenopus oocyte expression
system as the cloning assay. RNA will be extracted form tissue
sources of the cotransporter and purified for poly (A)+RNA. This
will be microinjected into oocytes. The second contransport system
to be investigated is the electrogenic Na-HCo3 cotransporter,
previously identified in the renal proximal tubule. There is
reason to believe it exists in ileal brush border membranes and is
central to ileal HCo3 secretion. We will study both intact ileal
epithelium and ileal brush border membrane vesicles to demonstrate
its presence. We will also inject mRNA from ileum and other
intestinal and renal sources into Xenopus oocyts to express the
cotransporter there. In addition to its importance in elucidating
normal epithelial physiology, the studies outlined may provide
insights into the pathophysiology of diarrheal and bronchial
diseases and how the latter can be ameliorated with specific drugs.
Statut | Terminé |
---|---|
Date de début/de fin réelle | 4/1/86 → 3/31/94 |
Financement
- National Institute of Diabetes and Digestive and Kidney Diseases
Keywords
- Fisiología
- Animales y zoología
Empreinte numérique
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