Regulators and interactomes of CaV1.2 in health and disease

Heart failure is associated with changes in the surface expression and regulation of the Calcium channel CaV1.2, which contributes to the exertional limitations and propensity for arrhythmia experienced by patients with this disease. Combining APEX proximity labeling with quantitative TMT SPS MS3, I will map the changing “neighborhood” of CaV1.2 in chronic heart failure. Using genetic models of APEX-defined candidate proteins, cellular electrophysiology, and an innovative transgenic mouse model engineered to assess CaV1.2 surface expression, I will gain deep insights into the molecular and cellular pathophysiology of heart failure.