A selective fluorescent probe for the detection of mercury (II) in aqueous media and its applications in living cells

Olimpo García-Beltrán; Natalia Mena; Tania A. Berríos; Enrique A. Castro; Bruce K. Cassels; Marco T. Núñez; Margarita E. Aliaga

doi:10.1016/j.tetlet.2012.09.111

A selective fluorescent probe for the detection of mercury (II) in aqueous media and its applications in living cells

Olimpo García-Beltrán, Natalia Mena, Tania A. Berríos, Enrique A. Castro, Bruce K. Cassels, Marco T. Núñez, Margarita E. Aliaga

Olimpo

Résultat de recherche › examen par les pairs

22 Citations (Scopus)

Résumé

In this Letter we present a new probe, 2-amino-3-hydroxy-2-(hydroxymethyl) propyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate (PMR), which can reversibly detect mercuric ions (Hg ²⁺) in HEPES buffer under physiological conditions. Possible interference with other analytes was examined. PMR displays a highly selective decrease of its fluorescence at 460 nm when it reacts with Hg ²⁺. Interestingly, the probe can also be used as a fluorescent turn-on sensor for biologically relevant thiols such as glutathione and cysteine. PMR can be used to determine mercury in living cells.

Langue d'origine	English
Pages (de-à)	6598-6601
Nombre de pages	4
Journal	Tetrahedron Letters
Volume	53
Numéro de publication	48
DOI	https://doi.org/10.1016/j.tetlet.2012.09.111
Statut de publication	Published - nov. 28 2012

Financement

This work was supported by the Millennium Scientific Initiative (Grant P05-001-F ) and by Fondecyt Grant # 11090115 . The authors are grateful to Prof. Frank H. Quina for the use of spectroscopic facilities in the Institute of Chemistry and the USP Research Consortium for Photochemical Technology (NAP-PhotoTech), University of São Paulo, São Paulo, Brazil.

Bailleurs de fonds	Numéro du bailleur de fonds
Fondo Nacional de Desarrollo Científico, Tecnológico y de Innovación Tecnológica	11090115

ASJC Scopus Subject Areas

Biochemistry
Drug Discovery
Organic Chemistry

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10.1016/j.tetlet.2012.09.111

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title = "A selective fluorescent probe for the detection of mercury (II) in aqueous media and its applications in living cells",

abstract = "In this Letter we present a new probe, 2-amino-3-hydroxy-2-(hydroxymethyl) propyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate (PMR), which can reversibly detect mercuric ions (Hg 2+) in HEPES buffer under physiological conditions. Possible interference with other analytes was examined. PMR displays a highly selective decrease of its fluorescence at 460 nm when it reacts with Hg 2+. Interestingly, the probe can also be used as a fluorescent turn-on sensor for biologically relevant thiols such as glutathione and cysteine. PMR can be used to determine mercury in living cells.",

author = "Olimpo Garc{\'i}a-Beltr{\'a}n and Natalia Mena and Berr{\'i}os, {Tania A.} and Castro, {Enrique A.} and Cassels, {Bruce K.} and N{\'u}{\~n}ez, {Marco T.} and Aliaga, {Margarita E.}",

note = "Funding Information: This work was supported by the Millennium Scientific Initiative (Grant P05-001-F ) and by Fondecyt Grant # 11090115 . The authors are grateful to Prof. Frank H. Quina for the use of spectroscopic facilities in the Institute of Chemistry and the USP Research Consortium for Photochemical Technology (NAP-PhotoTech), University of S{\~a}o Paulo, S{\~a}o Paulo, Brazil. ",

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T1 - A selective fluorescent probe for the detection of mercury (II) in aqueous media and its applications in living cells

AU - García-Beltrán, Olimpo

AU - Mena, Natalia

AU - Berríos, Tania A.

AU - Castro, Enrique A.

AU - Cassels, Bruce K.

AU - Núñez, Marco T.

AU - Aliaga, Margarita E.

N1 - Funding Information: This work was supported by the Millennium Scientific Initiative (Grant P05-001-F ) and by Fondecyt Grant # 11090115 . The authors are grateful to Prof. Frank H. Quina for the use of spectroscopic facilities in the Institute of Chemistry and the USP Research Consortium for Photochemical Technology (NAP-PhotoTech), University of São Paulo, São Paulo, Brazil.

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Y1 - 2012/11/28

N2 - In this Letter we present a new probe, 2-amino-3-hydroxy-2-(hydroxymethyl) propyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate (PMR), which can reversibly detect mercuric ions (Hg 2+) in HEPES buffer under physiological conditions. Possible interference with other analytes was examined. PMR displays a highly selective decrease of its fluorescence at 460 nm when it reacts with Hg 2+. Interestingly, the probe can also be used as a fluorescent turn-on sensor for biologically relevant thiols such as glutathione and cysteine. PMR can be used to determine mercury in living cells.

AB - In this Letter we present a new probe, 2-amino-3-hydroxy-2-(hydroxymethyl) propyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate (PMR), which can reversibly detect mercuric ions (Hg 2+) in HEPES buffer under physiological conditions. Possible interference with other analytes was examined. PMR displays a highly selective decrease of its fluorescence at 460 nm when it reacts with Hg 2+. Interestingly, the probe can also be used as a fluorescent turn-on sensor for biologically relevant thiols such as glutathione and cysteine. PMR can be used to determine mercury in living cells.

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A selective fluorescent probe for the detection of mercury (II) in aqueous media and its applications in living cells

Résumé

Financement

ASJC Scopus Subject Areas

Accès au document

Autres fichiers et liens

Empreinte numérique

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