TY - JOUR
T1 - Comparative Studies on Mammalian and Yeast Phenylalanine Transfer Ribonucleic Acids
AU - Fink, Louis M.
AU - Lanks, Karl W.
AU - Goto, Tamotsu
AU - Weinstein, I. Bernard
PY - 1971/5/1
Y1 - 1971/5/1
N2 - Rat liver tRNAPhe displays a high affinity for benzoylated DEAE-cellulose, thereby providing a simple method for its purification. This property is due to the presence of a fluorescent and hydrophobic nucleotide, “YR,” similar to the nucleotide “Y” present in tRNAphe obtained from yeast. Phenylalanine tRNA from adult rat liver, fetal fat liver, rabbit reticulocytes, Novikoff hepatoma, rat liver nuclei, and rat liver mitochondria exhibited similar chromatographic behavior suggesting that this nucleotide (or closely related substances) is widely distributed in mammalian systems. The fluorescent nucleotide from rat liver tRNAPhe appears to reside in the anticodon region and its fluorescence was environment sensitive in much the same mannrr as with yeast tRNPhe. However, fluorescence spectra of the purified tRNAs and thin-layer chromatography, absorption spectra and fluorescence excitation, and emission spectra of the purified fluorescent bases from yeast and rat liver tRNAphe suggest that these compounds have similar but not identical structures.
AB - Rat liver tRNAPhe displays a high affinity for benzoylated DEAE-cellulose, thereby providing a simple method for its purification. This property is due to the presence of a fluorescent and hydrophobic nucleotide, “YR,” similar to the nucleotide “Y” present in tRNAphe obtained from yeast. Phenylalanine tRNA from adult rat liver, fetal fat liver, rabbit reticulocytes, Novikoff hepatoma, rat liver nuclei, and rat liver mitochondria exhibited similar chromatographic behavior suggesting that this nucleotide (or closely related substances) is widely distributed in mammalian systems. The fluorescent nucleotide from rat liver tRNAPhe appears to reside in the anticodon region and its fluorescence was environment sensitive in much the same mannrr as with yeast tRNPhe. However, fluorescence spectra of the purified tRNAs and thin-layer chromatography, absorption spectra and fluorescence excitation, and emission spectra of the purified fluorescent bases from yeast and rat liver tRNAphe suggest that these compounds have similar but not identical structures.
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U2 - 10.1021/bi00786a022
DO - 10.1021/bi00786a022
M3 - Article
C2 - 4327495
AN - SCOPUS:0015219594
SN - 0006-2960
VL - 10
SP - 1873
EP - 1878
JO - Biochemistry
JF - Biochemistry
IS - 10
ER -