Zahir, H., Dehghani, B., Yuan, X., Chinenov, Y., Kim, C., Burge, A., Bandhari, R., Nemirov, D., Fava, P., Moley, P., Potter, H., Nguyen, J., Halpern, B., Donlin, L., Ivashkiv, L., Rodeo, S., & Otero, M. (2021). In vitro responses to platelet-rich-plasma are associated with variable clinical outcomes in patients with knee osteoarthritis. Scientific Reports, 11(1), Article 11493. https://doi.org/10.1038/s41598-021-90174-x
In vitro responses to platelet-rich-plasma are associated with variable clinical outcomes in patients with knee osteoarthritis. / Zahir, Habib; Dehghani, Bijan; Yuan, Xiaoning et al.
Dans:
Scientific Reports, Vol. 11, N° 1, 11493, 12.2021.
Résultat de recherche › examen par les pairs
Zahir, H, Dehghani, B, Yuan, X, Chinenov, Y, Kim, C, Burge, A, Bandhari, R, Nemirov, D, Fava, P, Moley, P, Potter, H, Nguyen, J, Halpern, B, Donlin, L, Ivashkiv, L, Rodeo, S & Otero, M 2021, 'In vitro responses to platelet-rich-plasma are associated with variable clinical outcomes in patients with knee osteoarthritis', Scientific Reports, vol. 11, n° 1, 11493. https://doi.org/10.1038/s41598-021-90174-x
Zahir H, Dehghani B, Yuan X, Chinenov Y, Kim C, Burge A et al. In vitro responses to platelet-rich-plasma are associated with variable clinical outcomes in patients with knee osteoarthritis. Scientific Reports. 2021 déc.;11(1):11493. doi: 10.1038/s41598-021-90174-x
Zahir, Habib ; Dehghani, Bijan ; Yuan, Xiaoning et al. / In vitro responses to platelet-rich-plasma are associated with variable clinical outcomes in patients with knee osteoarthritis. Dans: Scientific Reports. 2021 ; Vol. 11, N° 1.
@article{eeff25fb27314dcbab74679ec944e3c0,
title = "In vitro responses to platelet-rich-plasma are associated with variable clinical outcomes in patients with knee osteoarthritis",
abstract = "Autologous blood-derived products such as platelet-rich plasma (PRP) are widely used to treat musculoskeletal conditions, including knee osteoarthritis (OA). However, the clinical outcomes after PRP administration are often variable, and there is limited information about the specific characteristics of PRP that impact bioactivity and clinical responses. In this study, we aimed to develop an integrative workflow to evaluate responses to PRP in vitro, and to assess if the in vitro responses to PRP are associated with the PRP composition and clinical outcomes in patients with knee OA. To do this, we used a coculture system of macrophages and fibroblasts paired with transcriptomic analyses to comprehensively characterize the modulation of inflammatory responses by PRP in vitro. Relying on patient-reported outcomes and achievement of minimal clinically important differences in OA patients receiving PRP injections, we identified responders and non-responders to the treatment. Comparisons of PRP from these patient groups allowed us to identify differences in the composition and in vitro activity of PRP. We believe that our integrative workflow may enable the development of targeted approaches that rely on PRP and other orthobiologics to treat musculoskeletal pathologies.",
author = "Habib Zahir and Bijan Dehghani and Xiaoning Yuan and Yurii Chinenov and Christine Kim and Alissa Burge and Reyna Bandhari and Daniel Nemirov and Patrick Fava and Peter Moley and Hollis Potter and Joseph Nguyen and Brian Halpern and Laura Donlin and Lionel Ivashkiv and Scott Rodeo and Miguel Otero",
note = "Publisher Copyright: {\textcopyright} 2021, The Author(s).",
year = "2021",
month = dec,
doi = "10.1038/s41598-021-90174-x",
language = "English",
volume = "11",
journal = "Scientific Reports",
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T1 - In vitro responses to platelet-rich-plasma are associated with variable clinical outcomes in patients with knee osteoarthritis
AU - Zahir, Habib
AU - Dehghani, Bijan
AU - Yuan, Xiaoning
AU - Chinenov, Yurii
AU - Kim, Christine
AU - Burge, Alissa
AU - Bandhari, Reyna
AU - Nemirov, Daniel
AU - Fava, Patrick
AU - Moley, Peter
AU - Potter, Hollis
AU - Nguyen, Joseph
AU - Halpern, Brian
AU - Donlin, Laura
AU - Ivashkiv, Lionel
AU - Rodeo, Scott
AU - Otero, Miguel
N1 - Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Autologous blood-derived products such as platelet-rich plasma (PRP) are widely used to treat musculoskeletal conditions, including knee osteoarthritis (OA). However, the clinical outcomes after PRP administration are often variable, and there is limited information about the specific characteristics of PRP that impact bioactivity and clinical responses. In this study, we aimed to develop an integrative workflow to evaluate responses to PRP in vitro, and to assess if the in vitro responses to PRP are associated with the PRP composition and clinical outcomes in patients with knee OA. To do this, we used a coculture system of macrophages and fibroblasts paired with transcriptomic analyses to comprehensively characterize the modulation of inflammatory responses by PRP in vitro. Relying on patient-reported outcomes and achievement of minimal clinically important differences in OA patients receiving PRP injections, we identified responders and non-responders to the treatment. Comparisons of PRP from these patient groups allowed us to identify differences in the composition and in vitro activity of PRP. We believe that our integrative workflow may enable the development of targeted approaches that rely on PRP and other orthobiologics to treat musculoskeletal pathologies.
AB - Autologous blood-derived products such as platelet-rich plasma (PRP) are widely used to treat musculoskeletal conditions, including knee osteoarthritis (OA). However, the clinical outcomes after PRP administration are often variable, and there is limited information about the specific characteristics of PRP that impact bioactivity and clinical responses. In this study, we aimed to develop an integrative workflow to evaluate responses to PRP in vitro, and to assess if the in vitro responses to PRP are associated with the PRP composition and clinical outcomes in patients with knee OA. To do this, we used a coculture system of macrophages and fibroblasts paired with transcriptomic analyses to comprehensively characterize the modulation of inflammatory responses by PRP in vitro. Relying on patient-reported outcomes and achievement of minimal clinically important differences in OA patients receiving PRP injections, we identified responders and non-responders to the treatment. Comparisons of PRP from these patient groups allowed us to identify differences in the composition and in vitro activity of PRP. We believe that our integrative workflow may enable the development of targeted approaches that rely on PRP and other orthobiologics to treat musculoskeletal pathologies.
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