In vivo and in vitro evidence of basic fibroblast growth factor action in mouse mammary gland development

Sergio Lavandero; Andrés Chappuzeau; Mario Sapag-Hagar; Takami Oka

doi:10.1016/S0014-5793(98)01370-2

In vivo and in vitro evidence of basic fibroblast growth factor action in mouse mammary gland development

Sergio Lavandero, Andrés Chappuzeau, Mario Sapag-Hagar, Takami Oka

Universidad de Chile

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Basic fibroblast growth factor (bFGF) stimulated [³H]thymidine incorporation at all stages of development, although the magnitude of this effect was the greatest in cells derived from pregnant mice. Cells primed with insulin and bFGF synthesized more casein than cells not exposed to either hormone. bFGF inhibited casein synthesis and decreased the amounts of β-casein and α-lactalbumin transcripts in cells from pregnant animals simultaneously incubated with insulin, hydrocortisone and prolactin. bFGF content in mammary gland increased with puberty and pregnancy, but decreased markedly in lactation; the number of bFGF receptors in epithelial cells changed in parallel. These data suggest that bFGF may have a physiological role both in stimulating growth and in inhibiting functional differentiation of normal mouse mammary epithelial cells. Copyright (C) 1998 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	351-356
Number of pages	6
Journal	FEBS Letters
Volume	439
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(98)01370-2
Publication status	Published - Nov 20 1998

Bibliographical note

Funding Information:
We wish to thank Dr P. Qasba, NCI, National Institutes of Health, for the kind gift of the mouse α-lactalbumin cDNA clone and Dr C. George-Nascimento, Chiron Corp., for the donation of recombinant human bFGF. This work was supported in part by Grants FONDECYT 91-0879 and DTI B-3113-9013 (S.L.). S.L. is the recipient of a Fellowship from Fundación Andes (Chile). We also thank Dr C.I. Pogson for his help in the revision of the manuscript.

Funding

We wish to thank Dr P. Qasba, NCI, National Institutes of Health, for the kind gift of the mouse α-lactalbumin cDNA clone and Dr C. George-Nascimento, Chiron Corp., for the donation of recombinant human bFGF. This work was supported in part by Grants FONDECYT 91-0879 and DTI B-3113-9013 (S.L.). S.L. is the recipient of a Fellowship from Fundación Andes (Chile). We also thank Dr C.I. Pogson for his help in the revision of the manuscript.

Funders	Funder number
Fondo Nacional de Desarrollo Científico y Tecnológico	DTI B-3113-9013, 91-0879

ASJC Scopus Subject Areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "In vivo and in vitro evidence of basic fibroblast growth factor action in mouse mammary gland development",

abstract = "Basic fibroblast growth factor (bFGF) stimulated [3H]thymidine incorporation at all stages of development, although the magnitude of this effect was the greatest in cells derived from pregnant mice. Cells primed with insulin and bFGF synthesized more casein than cells not exposed to either hormone. bFGF inhibited casein synthesis and decreased the amounts of β-casein and α-lactalbumin transcripts in cells from pregnant animals simultaneously incubated with insulin, hydrocortisone and prolactin. bFGF content in mammary gland increased with puberty and pregnancy, but decreased markedly in lactation; the number of bFGF receptors in epithelial cells changed in parallel. These data suggest that bFGF may have a physiological role both in stimulating growth and in inhibiting functional differentiation of normal mouse mammary epithelial cells. Copyright (C) 1998 Federation of European Biochemical Societies.",

author = "Sergio Lavandero and Andr{\'e}s Chappuzeau and Mario Sapag-Hagar and Takami Oka",

note = "Funding Information: We wish to thank Dr P. Qasba, NCI, National Institutes of Health, for the kind gift of the mouse α-lactalbumin cDNA clone and Dr C. George-Nascimento, Chiron Corp., for the donation of recombinant human bFGF. This work was supported in part by Grants FONDECYT 91-0879 and DTI B-3113-9013 (S.L.). S.L. is the recipient of a Fellowship from Fundaci{\'o}n Andes (Chile). We also thank Dr C.I. Pogson for his help in the revision of the manuscript.",

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AU - Oka, Takami

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N2 - Basic fibroblast growth factor (bFGF) stimulated [3H]thymidine incorporation at all stages of development, although the magnitude of this effect was the greatest in cells derived from pregnant mice. Cells primed with insulin and bFGF synthesized more casein than cells not exposed to either hormone. bFGF inhibited casein synthesis and decreased the amounts of β-casein and α-lactalbumin transcripts in cells from pregnant animals simultaneously incubated with insulin, hydrocortisone and prolactin. bFGF content in mammary gland increased with puberty and pregnancy, but decreased markedly in lactation; the number of bFGF receptors in epithelial cells changed in parallel. These data suggest that bFGF may have a physiological role both in stimulating growth and in inhibiting functional differentiation of normal mouse mammary epithelial cells. Copyright (C) 1998 Federation of European Biochemical Societies.

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In vivo and in vitro evidence of basic fibroblast growth factor action in mouse mammary gland development

Abstract

Bibliographical note

Funding

ASJC Scopus Subject Areas

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