TY - JOUR
T1 - In vivo and in vitro evidence of basic fibroblast growth factor action in mouse mammary gland development
AU - Lavandero, Sergio
AU - Chappuzeau, Andrés
AU - Sapag-Hagar, Mario
AU - Oka, Takami
N1 - Funding Information:
We wish to thank Dr P. Qasba, NCI, National Institutes of Health, for the kind gift of the mouse α-lactalbumin cDNA clone and Dr C. George-Nascimento, Chiron Corp., for the donation of recombinant human bFGF. This work was supported in part by Grants FONDECYT 91-0879 and DTI B-3113-9013 (S.L.). S.L. is the recipient of a Fellowship from Fundación Andes (Chile). We also thank Dr C.I. Pogson for his help in the revision of the manuscript.
PY - 1998/11/20
Y1 - 1998/11/20
N2 - Basic fibroblast growth factor (bFGF) stimulated [3H]thymidine incorporation at all stages of development, although the magnitude of this effect was the greatest in cells derived from pregnant mice. Cells primed with insulin and bFGF synthesized more casein than cells not exposed to either hormone. bFGF inhibited casein synthesis and decreased the amounts of β-casein and α-lactalbumin transcripts in cells from pregnant animals simultaneously incubated with insulin, hydrocortisone and prolactin. bFGF content in mammary gland increased with puberty and pregnancy, but decreased markedly in lactation; the number of bFGF receptors in epithelial cells changed in parallel. These data suggest that bFGF may have a physiological role both in stimulating growth and in inhibiting functional differentiation of normal mouse mammary epithelial cells. Copyright (C) 1998 Federation of European Biochemical Societies.
AB - Basic fibroblast growth factor (bFGF) stimulated [3H]thymidine incorporation at all stages of development, although the magnitude of this effect was the greatest in cells derived from pregnant mice. Cells primed with insulin and bFGF synthesized more casein than cells not exposed to either hormone. bFGF inhibited casein synthesis and decreased the amounts of β-casein and α-lactalbumin transcripts in cells from pregnant animals simultaneously incubated with insulin, hydrocortisone and prolactin. bFGF content in mammary gland increased with puberty and pregnancy, but decreased markedly in lactation; the number of bFGF receptors in epithelial cells changed in parallel. These data suggest that bFGF may have a physiological role both in stimulating growth and in inhibiting functional differentiation of normal mouse mammary epithelial cells. Copyright (C) 1998 Federation of European Biochemical Societies.
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U2 - 10.1016/S0014-5793(98)01370-2
DO - 10.1016/S0014-5793(98)01370-2
M3 - Article
C2 - 9845352
AN - SCOPUS:0031762405
SN - 0014-5793
VL - 439
SP - 351
EP - 356
JO - FEBS Letters
JF - FEBS Letters
IS - 3
ER -