Laboratory diagnosis of infection status in infants perinatally exposed to human immunodeficiency virus type 1

Morris O. Paul; Surya Tetali; Martin L. Lesser; Elaine J. Abrams; Xue Ping Wang; Regina Kowalski; Mahrukh Bamji; Barbara Napolitano; Leslie Gulick; Saroj Bakshi; Savita Pahwa

doi:10.1093/infdis/173.1.68

Laboratory diagnosis of infection status in infants perinatally exposed to human immunodeficiency virus type 1

Morris O. Paul, Surya Tetali, Martin L. Lesser, Elaine J. Abrams, Xue Ping Wang, Regina Kowalski, Mahrukh Bamji, Barbara Napolitano, Leslie Gulick, Saroj Bakshi, Savita Pahwa

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Accurate and timely diagnosis of infection status in infants born to women infected with human immunodeficiency virus (HIV) is of paramount importance. The comparative accuracy of five diagnostic decision rules was evaluated in 208 HIV-exposed infants (32 infected, 176 uninfected) based on laboratory testing during the first 6 months of life. Diagnostic rules A and B, which required single blood samples analyzed by culture and polymerase chain reaction (PCR) (rule A) or culture, PCR, and p24 antigen detection (rule B) were more prone to incorrect diagnoses than were rules requiring 2 blood samples analyzed by a single assay (rule C) or combinations of culture and PCR (rules D and E). Rule D, which used PCR as the initial test, established the most useful algorithm: a positive PCR result followed by a positive culture in the second sample confirmed infected status, while two consecutive negative PCR results reconfirmed as negative at 6 months of age established uninfected status.

Original language	English
Pages (from-to)	68-76
Number of pages	9
Journal	Journal of Infectious Diseases
Volume	173
Issue number	1
DOIs	https://doi.org/10.1093/infdis/173.1.68
Publication status	Published - 1996

Bibliographical note

Funding Information:
Received 4 April 1995; revised 14 August 1995. Presented in part: American Pediatric Society annual meeting, Seattle, May 1994. Informed consent was obtained from parents and guardians and human experimentation guidelines of the US Department of Health and Human Services and those of North Shore University Hospital-Cornell University Medical College were followed in the conduct of clinical research. Grant support: National Institutes of Health (HD-26606). Reprints or correspondence: Dr. Savita Pahwa,Div. of Pediatric Immunology, North Shore University Hospital-Cornell University Medical College, 350 Community Dr., Manhasset, NY 11030.

Funding

Received 4 April 1995; revised 14 August 1995. Presented in part: American Pediatric Society annual meeting, Seattle, May 1994. Informed consent was obtained from parents and guardians and human experimentation guidelines of the US Department of Health and Human Services and those of North Shore University Hospital-Cornell University Medical College were followed in the conduct of clinical research. Grant support: National Institutes of Health (HD-26606). Reprints or correspondence: Dr. Savita Pahwa,Div. of Pediatric Immunology, North Shore University Hospital-Cornell University Medical College, 350 Community Dr., Manhasset, NY 11030.

Funders	Funder number
National Institutes of Health
Eunice Kennedy Shriver National Institute of Child Health and Human Development	R01HD026606

ASJC Scopus Subject Areas

Immunology and Allergy
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1093/infdis/173.1.68

Cite this

@article{a49bc61ebfae40be914daf6f98495652,

title = "Laboratory diagnosis of infection status in infants perinatally exposed to human immunodeficiency virus type 1",

abstract = "Accurate and timely diagnosis of infection status in infants born to women infected with human immunodeficiency virus (HIV) is of paramount importance. The comparative accuracy of five diagnostic decision rules was evaluated in 208 HIV-exposed infants (32 infected, 176 uninfected) based on laboratory testing during the first 6 months of life. Diagnostic rules A and B, which required single blood samples analyzed by culture and polymerase chain reaction (PCR) (rule A) or culture, PCR, and p24 antigen detection (rule B) were more prone to incorrect diagnoses than were rules requiring 2 blood samples analyzed by a single assay (rule C) or combinations of culture and PCR (rules D and E). Rule D, which used PCR as the initial test, established the most useful algorithm: a positive PCR result followed by a positive culture in the second sample confirmed infected status, while two consecutive negative PCR results reconfirmed as negative at 6 months of age established uninfected status.",

author = "Paul, {Morris O.} and Surya Tetali and Lesser, {Martin L.} and Abrams, {Elaine J.} and Wang, {Xue Ping} and Regina Kowalski and Mahrukh Bamji and Barbara Napolitano and Leslie Gulick and Saroj Bakshi and Savita Pahwa",

note = "Funding Information: Received 4 April 1995; revised 14 August 1995. Presented in part: American Pediatric Society annual meeting, Seattle, May 1994. Informed consent was obtained from parents and guardians and human experimentation guidelines of the US Department of Health and Human Services and those of North Shore University Hospital-Cornell University Medical College were followed in the conduct of clinical research. Grant support: National Institutes of Health (HD-26606). Reprints or correspondence: Dr. Savita Pahwa,Div. of Pediatric Immunology, North Shore University Hospital-Cornell University Medical College, 350 Community Dr., Manhasset, NY 11030.",

year = "1996",

doi = "10.1093/infdis/173.1.68",

language = "English",

volume = "173",

pages = "68--76",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

number = "1",

}

TY - JOUR

T1 - Laboratory diagnosis of infection status in infants perinatally exposed to human immunodeficiency virus type 1

AU - Paul, Morris O.

AU - Tetali, Surya

AU - Lesser, Martin L.

AU - Abrams, Elaine J.

AU - Wang, Xue Ping

AU - Kowalski, Regina

AU - Bamji, Mahrukh

AU - Napolitano, Barbara

AU - Gulick, Leslie

AU - Bakshi, Saroj

AU - Pahwa, Savita

N1 - Funding Information: Received 4 April 1995; revised 14 August 1995. Presented in part: American Pediatric Society annual meeting, Seattle, May 1994. Informed consent was obtained from parents and guardians and human experimentation guidelines of the US Department of Health and Human Services and those of North Shore University Hospital-Cornell University Medical College were followed in the conduct of clinical research. Grant support: National Institutes of Health (HD-26606). Reprints or correspondence: Dr. Savita Pahwa,Div. of Pediatric Immunology, North Shore University Hospital-Cornell University Medical College, 350 Community Dr., Manhasset, NY 11030.

PY - 1996

Y1 - 1996

N2 - Accurate and timely diagnosis of infection status in infants born to women infected with human immunodeficiency virus (HIV) is of paramount importance. The comparative accuracy of five diagnostic decision rules was evaluated in 208 HIV-exposed infants (32 infected, 176 uninfected) based on laboratory testing during the first 6 months of life. Diagnostic rules A and B, which required single blood samples analyzed by culture and polymerase chain reaction (PCR) (rule A) or culture, PCR, and p24 antigen detection (rule B) were more prone to incorrect diagnoses than were rules requiring 2 blood samples analyzed by a single assay (rule C) or combinations of culture and PCR (rules D and E). Rule D, which used PCR as the initial test, established the most useful algorithm: a positive PCR result followed by a positive culture in the second sample confirmed infected status, while two consecutive negative PCR results reconfirmed as negative at 6 months of age established uninfected status.

AB - Accurate and timely diagnosis of infection status in infants born to women infected with human immunodeficiency virus (HIV) is of paramount importance. The comparative accuracy of five diagnostic decision rules was evaluated in 208 HIV-exposed infants (32 infected, 176 uninfected) based on laboratory testing during the first 6 months of life. Diagnostic rules A and B, which required single blood samples analyzed by culture and polymerase chain reaction (PCR) (rule A) or culture, PCR, and p24 antigen detection (rule B) were more prone to incorrect diagnoses than were rules requiring 2 blood samples analyzed by a single assay (rule C) or combinations of culture and PCR (rules D and E). Rule D, which used PCR as the initial test, established the most useful algorithm: a positive PCR result followed by a positive culture in the second sample confirmed infected status, while two consecutive negative PCR results reconfirmed as negative at 6 months of age established uninfected status.

UR - http://www.scopus.com/inward/record.url?scp=19144362419&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=19144362419&partnerID=8YFLogxK

U2 - 10.1093/infdis/173.1.68

DO - 10.1093/infdis/173.1.68

M3 - Article

C2 - 8537684

AN - SCOPUS:19144362419

SN - 0022-1899

VL - 173

SP - 68

EP - 76

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

IS - 1

ER -

Laboratory diagnosis of infection status in infants perinatally exposed to human immunodeficiency virus type 1

Abstract

Bibliographical note

Funding

ASJC Scopus Subject Areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this