TY - JOUR
T1 - Laboratory diagnosis of infection status in infants perinatally exposed to human immunodeficiency virus type 1
AU - Paul, Morris O.
AU - Tetali, Surya
AU - Lesser, Martin L.
AU - Abrams, Elaine J.
AU - Wang, Xue Ping
AU - Kowalski, Regina
AU - Bamji, Mahrukh
AU - Napolitano, Barbara
AU - Gulick, Leslie
AU - Bakshi, Saroj
AU - Pahwa, Savita
N1 - Funding Information:
Received 4 April 1995; revised 14 August 1995. Presented in part: American Pediatric Society annual meeting, Seattle, May 1994. Informed consent was obtained from parents and guardians and human experimentation guidelines of the US Department of Health and Human Services and those of North Shore University Hospital-Cornell University Medical College were followed in the conduct of clinical research. Grant support: National Institutes of Health (HD-26606). Reprints or correspondence: Dr. Savita Pahwa,Div. of Pediatric Immunology, North Shore University Hospital-Cornell University Medical College, 350 Community Dr., Manhasset, NY 11030.
PY - 1996
Y1 - 1996
N2 - Accurate and timely diagnosis of infection status in infants born to women infected with human immunodeficiency virus (HIV) is of paramount importance. The comparative accuracy of five diagnostic decision rules was evaluated in 208 HIV-exposed infants (32 infected, 176 uninfected) based on laboratory testing during the first 6 months of life. Diagnostic rules A and B, which required single blood samples analyzed by culture and polymerase chain reaction (PCR) (rule A) or culture, PCR, and p24 antigen detection (rule B) were more prone to incorrect diagnoses than were rules requiring 2 blood samples analyzed by a single assay (rule C) or combinations of culture and PCR (rules D and E). Rule D, which used PCR as the initial test, established the most useful algorithm: a positive PCR result followed by a positive culture in the second sample confirmed infected status, while two consecutive negative PCR results reconfirmed as negative at 6 months of age established uninfected status.
AB - Accurate and timely diagnosis of infection status in infants born to women infected with human immunodeficiency virus (HIV) is of paramount importance. The comparative accuracy of five diagnostic decision rules was evaluated in 208 HIV-exposed infants (32 infected, 176 uninfected) based on laboratory testing during the first 6 months of life. Diagnostic rules A and B, which required single blood samples analyzed by culture and polymerase chain reaction (PCR) (rule A) or culture, PCR, and p24 antigen detection (rule B) were more prone to incorrect diagnoses than were rules requiring 2 blood samples analyzed by a single assay (rule C) or combinations of culture and PCR (rules D and E). Rule D, which used PCR as the initial test, established the most useful algorithm: a positive PCR result followed by a positive culture in the second sample confirmed infected status, while two consecutive negative PCR results reconfirmed as negative at 6 months of age established uninfected status.
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U2 - 10.1093/infdis/173.1.68
DO - 10.1093/infdis/173.1.68
M3 - Article
C2 - 8537684
AN - SCOPUS:19144362419
SN - 0022-1899
VL - 173
SP - 68
EP - 76
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 1
ER -
