Light-induced release of the cardioprotective peptide angiotensin-(1–9) from thermosensitive liposomes with gold nanoclusters

Julian Bejarano; Aldo Rojas; Andrea Ramírez-Sagredo; Ana L. Riveros; Francisco Morales-Zavala; Yvo Flores; Jaime A. Riquelme; Fanny Guzman; Eyleen Araya; Mario Chiong; María Paz Ocaranza; Javier O. Morales; María Gabriela Villamizar Sarmiento; Gina Sanchez; Sergio Lavandero; Marcelo J. Kogan

doi:10.1016/j.jconrel.2020.11.002

Light-induced release of the cardioprotective peptide angiotensin-(1–9) from thermosensitive liposomes with gold nanoclusters

Julian Bejarano, Aldo Rojas, Andrea Ramírez-Sagredo, Ana L. Riveros, Francisco Morales-Zavala, Yvo Flores, Jaime A. Riquelme, Fanny Guzman, Eyleen Araya, Mario Chiong, María Paz Ocaranza, Javier O. Morales, María Gabriela Villamizar Sarmiento, Gina Sanchez, Sergio Lavandero, Marcelo J. Kogan

Universidad de Chile

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Angiotensin-(1-9), a component of the non-canonical renin-angiotensin system, has a short half-life in blood. This peptide has shown to prevent and/or attenuate hypertension and cardiovascular remodeling. A controlled release of angiotensin-(1-9) is needed for its delivery to the heart. Our aim was to develop a drug delivery system for angiotensin-(1-9). Thermosensitive liposomes (LipoTherm) were prepared with gold nanoclusters (LipoTherm-AuNC) to increase the stability and reach a temporal and spatial control of angiotensin-(1-9) release. Encapsulation efficiencies of nearly 50% were achieved in LipoTherm, reaching a total angiotensin-(1-9) loading of around 180 μM. This angiotensin-(1–9)-loaded LipoTherm sized around 100 nm and exhibited a phase transition temperature of 43 °C. AuNC were grown on LipoTherm and the new hybrid nanosystem showed energy absorption in the near-infrared (NIR) wavelength range. By NIR laser irradiation, a controlled release of angiotensin-(1-9) was achieved from the LipoTherm-AuNC nanosystem. These nanosystems did not show any cytotoxic effect on cultured cardiomyocytes. Biological activity of angiotensin-(1-9) released from the LipoTherm-AuNC-based nanosystem was confirmed using an ex vivo Langendorff heart model.

Original language	English
Pages (from-to)	859-872
Number of pages	14
Journal	Journal of Controlled Release
Volume	328
DOIs	https://doi.org/10.1016/j.jconrel.2020.11.002
Publication status	Published - Dec 10 2020

Bibliographical note

Publisher Copyright:
© 2020 Elsevier B.V.

Funding

The authors received funding from Agencia Nacional de Investigación y Desarrollo (ANID), Chile: grants FONDAP 15130011 (to M.P.O., J.A.R., M.C., M.K. and S.L); Puente Pontificia Universidad Católica de Chile (033/2020 to M.P.O.), Fondecyt 1190623 (E.A) and 1200490 (to S.L.), Bayer AG (Program Grants4Targets ID 2017-08-2260 (to M.P.O., M.C. and S.L.), Anillo ACT192144 (to M.P.O., E.A, and J.M.), Fondecyt Postdoctoral Fellowship 3160323 (J.B) and FONDEQUIP EQM160157 and EQM170111. The authors received funding from Agencia Nacional de Investigaci?n y Desarrollo (ANID), Chile: grants FONDAP 15130011 (to M.P.O. J.A.R. M.C. M.K. and S.L); Puente Pontificia Universidad Cat?lica de Chile (033/2020 to M.P.O.), Fondecyt 1190623 (E.A) and 1200490 (to S.L.), Bayer AG (Program Grants4Targets ID 2017-08-2260 (to M.P.O. M.C. and S.L.), Anillo ACT192144 (to M.P.O. E.A, and J.M.), Fondecyt Postdoctoral Fellowship 3160323 (J.B) and FONDEQUIP EQM160157 and EQM170111.

Funders	Funder number
Agencia Nacional de Investigaci?n y Desarrollo
Agencia Nacional de Investigación y Desarrollo	FONDAP 15130011
FONDEQUIP	EQM160157, EQM170111
Puente Pontificia Universidad Cat?lica de Chile
Bayer	2017-08-2260, 3160323, ACT192144
Fondo Nacional de Desarrollo Científico y Tecnológico	1200490, 1190623
Pontificia Universidad Católica de Chile	033/2020

ASJC Scopus Subject Areas

Pharmaceutical Science

Access to Document

10.1016/j.jconrel.2020.11.002

Cite this

Bejarano, J., Rojas, A., Ramírez-Sagredo, A., Riveros, A. L., Morales-Zavala, F., Flores, Y., Riquelme, J. A., Guzman, F., Araya, E., Chiong, M., Ocaranza, M. P., Morales, J. O., Villamizar Sarmiento, M. G., Sanchez, G., Lavandero, S., & Kogan, M. J. (2020). Light-induced release of the cardioprotective peptide angiotensin-(1–9) from thermosensitive liposomes with gold nanoclusters. Journal of Controlled Release, 328, 859-872. https://doi.org/10.1016/j.jconrel.2020.11.002

Bejarano, J, Rojas, A, Ramírez-Sagredo, A, Riveros, AL, Morales-Zavala, F, Flores, Y, Riquelme, JA, Guzman, F, Araya, E, Chiong, M, Ocaranza, MP, Morales, JO, Villamizar Sarmiento, MG, Sanchez, G, Lavandero, S & Kogan, MJ 2020, 'Light-induced release of the cardioprotective peptide angiotensin-(1–9) from thermosensitive liposomes with gold nanoclusters', Journal of Controlled Release, vol. 328, pp. 859-872. https://doi.org/10.1016/j.jconrel.2020.11.002

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abstract = "Angiotensin-(1-9), a component of the non-canonical renin-angiotensin system, has a short half-life in blood. This peptide has shown to prevent and/or attenuate hypertension and cardiovascular remodeling. A controlled release of angiotensin-(1-9) is needed for its delivery to the heart. Our aim was to develop a drug delivery system for angiotensin-(1-9). Thermosensitive liposomes (LipoTherm) were prepared with gold nanoclusters (LipoTherm-AuNC) to increase the stability and reach a temporal and spatial control of angiotensin-(1-9) release. Encapsulation efficiencies of nearly 50% were achieved in LipoTherm, reaching a total angiotensin-(1-9) loading of around 180 μM. This angiotensin-(1–9)-loaded LipoTherm sized around 100 nm and exhibited a phase transition temperature of 43 °C. AuNC were grown on LipoTherm and the new hybrid nanosystem showed energy absorption in the near-infrared (NIR) wavelength range. By NIR laser irradiation, a controlled release of angiotensin-(1-9) was achieved from the LipoTherm-AuNC nanosystem. These nanosystems did not show any cytotoxic effect on cultured cardiomyocytes. Biological activity of angiotensin-(1-9) released from the LipoTherm-AuNC-based nanosystem was confirmed using an ex vivo Langendorff heart model.",

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AU - Bejarano, Julian

AU - Rojas, Aldo

AU - Ramírez-Sagredo, Andrea

AU - Riveros, Ana L.

AU - Morales-Zavala, Francisco

AU - Flores, Yvo

AU - Riquelme, Jaime A.

AU - Guzman, Fanny

AU - Araya, Eyleen

AU - Chiong, Mario

AU - Ocaranza, María Paz

AU - Morales, Javier O.

AU - Villamizar Sarmiento, María Gabriela

AU - Sanchez, Gina

AU - Lavandero, Sergio

AU - Kogan, Marcelo J.

PY - 2020/12/10

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N2 - Angiotensin-(1-9), a component of the non-canonical renin-angiotensin system, has a short half-life in blood. This peptide has shown to prevent and/or attenuate hypertension and cardiovascular remodeling. A controlled release of angiotensin-(1-9) is needed for its delivery to the heart. Our aim was to develop a drug delivery system for angiotensin-(1-9). Thermosensitive liposomes (LipoTherm) were prepared with gold nanoclusters (LipoTherm-AuNC) to increase the stability and reach a temporal and spatial control of angiotensin-(1-9) release. Encapsulation efficiencies of nearly 50% were achieved in LipoTherm, reaching a total angiotensin-(1-9) loading of around 180 μM. This angiotensin-(1–9)-loaded LipoTherm sized around 100 nm and exhibited a phase transition temperature of 43 °C. AuNC were grown on LipoTherm and the new hybrid nanosystem showed energy absorption in the near-infrared (NIR) wavelength range. By NIR laser irradiation, a controlled release of angiotensin-(1-9) was achieved from the LipoTherm-AuNC nanosystem. These nanosystems did not show any cytotoxic effect on cultured cardiomyocytes. Biological activity of angiotensin-(1-9) released from the LipoTherm-AuNC-based nanosystem was confirmed using an ex vivo Langendorff heart model.

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Light-induced release of the cardioprotective peptide angiotensin-(1–9) from thermosensitive liposomes with gold nanoclusters

Abstract

Bibliographical note

Funding

ASJC Scopus Subject Areas

Access to Document

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