Light-induced release of the cardioprotective peptide angiotensin-(1–9) from thermosensitive liposomes with gold nanoclusters

Julian Bejarano, Aldo Rojas, Andrea Ramírez-Sagredo, Ana L. Riveros, Francisco Morales-Zavala, Yvo Flores, Jaime A. Riquelme, Fanny Guzman, Eyleen Araya, Mario Chiong, María Paz Ocaranza, Javier O. Morales, María Gabriela Villamizar Sarmiento, Gina Sanchez, Sergio Lavandero, Marcelo J. Kogan

Producción científicarevisión exhaustiva

10 Citas (Scopus)

Resumen

Angiotensin-(1-9), a component of the non-canonical renin-angiotensin system, has a short half-life in blood. This peptide has shown to prevent and/or attenuate hypertension and cardiovascular remodeling. A controlled release of angiotensin-(1-9) is needed for its delivery to the heart. Our aim was to develop a drug delivery system for angiotensin-(1-9). Thermosensitive liposomes (LipoTherm) were prepared with gold nanoclusters (LipoTherm-AuNC) to increase the stability and reach a temporal and spatial control of angiotensin-(1-9) release. Encapsulation efficiencies of nearly 50% were achieved in LipoTherm, reaching a total angiotensin-(1-9) loading of around 180 μM. This angiotensin-(1–9)-loaded LipoTherm sized around 100 nm and exhibited a phase transition temperature of 43 °C. AuNC were grown on LipoTherm and the new hybrid nanosystem showed energy absorption in the near-infrared (NIR) wavelength range. By NIR laser irradiation, a controlled release of angiotensin-(1-9) was achieved from the LipoTherm-AuNC nanosystem. These nanosystems did not show any cytotoxic effect on cultured cardiomyocytes. Biological activity of angiotensin-(1-9) released from the LipoTherm-AuNC-based nanosystem was confirmed using an ex vivo Langendorff heart model.

Idioma originalEnglish
Páginas (desde-hasta)859-872
Número de páginas14
PublicaciónJournal of Controlled Release
Volumen328
DOI
EstadoPublished - dic. 10 2020

Financiación

The authors received funding from Agencia Nacional de Investigación y Desarrollo (ANID), Chile: grants FONDAP 15130011 (to M.P.O., J.A.R., M.C., M.K. and S.L); Puente Pontificia Universidad Católica de Chile (033/2020 to M.P.O.), Fondecyt 1190623 (E.A) and 1200490 (to S.L.), Bayer AG (Program Grants4Targets ID 2017-08-2260 (to M.P.O., M.C. and S.L.), Anillo ACT192144 (to M.P.O., E.A, and J.M.), Fondecyt Postdoctoral Fellowship 3160323 (J.B) and FONDEQUIP EQM160157 and EQM170111. The authors received funding from Agencia Nacional de Investigaci?n y Desarrollo (ANID), Chile: grants FONDAP 15130011 (to M.P.O. J.A.R. M.C. M.K. and S.L); Puente Pontificia Universidad Cat?lica de Chile (033/2020 to M.P.O.), Fondecyt 1190623 (E.A) and 1200490 (to S.L.), Bayer AG (Program Grants4Targets ID 2017-08-2260 (to M.P.O. M.C. and S.L.), Anillo ACT192144 (to M.P.O. E.A, and J.M.), Fondecyt Postdoctoral Fellowship 3160323 (J.B) and FONDEQUIP EQM160157 and EQM170111.

FinanciadoresNúmero del financiador
Agencia Nacional de Investigaci?n y Desarrollo
Agencia Nacional de Investigación y DesarrolloFONDAP 15130011
FONDEQUIPEQM160157, EQM170111
Puente Pontificia Universidad Cat?lica de Chile
Bayer2017-08-2260, 3160323, ACT192144
Fondo Nacional de Desarrollo Científico y Tecnológico1200490, 1190623
Pontificia Universidad Católica de Chile033/2020

    ASJC Scopus Subject Areas

    • Pharmaceutical Science

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