Abstract
Continuous-flow resonance Raman experiments carried out in bacteriorhodopsin show that the exchange of a deuteron on the Schiff base with a proton takes place in times shorter than 3 ms. Exchange mechanisms based on a base-catalyzed deprotonation followed by reprotonation of the Schiff base are excluded. A mechanism is suggested in which a water molecule interacts directly with the Schiff base deuteron in a concerted exchange mechanism. It appears that in the dark, the binding site is more accessible to neutral water molecules than to charged protons.
Original language | English |
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Pages (from-to) | 275-279 |
Number of pages | 5 |
Journal | Biophysical Journal |
Volume | 33 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1981 |
Bibliographical note
Funding Information:This work was supported in part by the National Science Foundation (PCM79-02683), by the National Institutes of Health (EY03142), by a City University PSC-BHE Faculty Award at the City College, and by the National Science Foundation (PCM80-1093 1) at the University of Illinois.
Funding
This work was supported in part by the National Science Foundation (PCM79-02683), by the National Institutes of Health (EY03142), by a City University PSC-BHE Faculty Award at the City College, and by the National Science Foundation (PCM80-1093 1) at the University of Illinois.
Funders | Funder number |
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City College | PCM80-1093 1 |
City University | |
National Science Foundation | PCM79-02683 |
National Institutes of Health | EY03142 |
ASJC Scopus Subject Areas
- Biophysics