On the mechanism of hydrogen-deuterium exchange in bacteriorhodopsin

A. G. Doukas, A. Pande, T. Suzuki, R. H. Callender, B. Honig, M. Ottolenghi

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32 Citas (Scopus)

Resumen

Continuous-flow resonance Raman experiments carried out in bacteriorhodopsin show that the exchange of a deuteron on the Schiff base with a proton takes place in times shorter than 3 ms. Exchange mechanisms based on a base-catalyzed deprotonation followed by reprotonation of the Schiff base are excluded. A mechanism is suggested in which a water molecule interacts directly with the Schiff base deuteron in a concerted exchange mechanism. It appears that in the dark, the binding site is more accessible to neutral water molecules than to charged protons.

Idioma originalEnglish
Páginas (desde-hasta)275-279
Número de páginas5
PublicaciónBiophysical Journal
Volumen33
N.º2
DOI
EstadoPublished - 1981

Financiación

FinanciadoresNúmero del financiador
City CollegePCM80-1093 1
City University
National Science FoundationPCM79-02683
National Institutes of HealthEY03142

    ASJC Scopus Subject Areas

    • Biophysics

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