Tumor promoters inhibit spontaneous and induced differentiation of murine erythroleukemia cells in culture

H. Yamasaki, E. Fibach, U. Nudel, I. B. Weinstein, R. A. Rifkind, P. A. Marks

Research output: Contribution to journalArticlepeer-review

241 Citations (Scopus)

Abstract

Addition of the potent tumor promoter, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), to murine erythroleukemia cell lines in suspension cultures inhibited both spontaneous differentiation and differentiation induced by hexamethylene bisacetamide (HMBA), dimethyl sulfoxide, or butyric acid. Inhibition was unrelated to cytotoxicity and was reversible. When several plant diterpenes were tested, there was a positive correlation between tumor-promoting activity and inhibition of differentiation. TPA inhibited HMBA-induced differentiation only if added prior to the time of commitment to differentiation, as assayed by scoring for differentiation after transfer of cells from HMBA to fresh medium without HMBA. TPA-mediated inhibition of differentiation was associated with a decrease in globin mRNA accumulation.

Original languageEnglish
Pages (from-to)3451-3455
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume74
Issue number8
DOIs
Publication statusPublished - 1977

ASJC Scopus Subject Areas

  • General

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