Tumor promoters inhibit spontaneous and induced differentiation of murine erythroleukemia cells in culture

H. Yamasaki; E. Fibach; U. Nudel; I. B. Weinstein; R. A. Rifkind; P. A. Marks

doi:10.1073/pnas.74.8.3451

Tumor promoters inhibit spontaneous and induced differentiation of murine erythroleukemia cells in culture

H. Yamasaki, E. Fibach, U. Nudel, I. B. Weinstein, R. A. Rifkind, P. A. Marks

Vagelos College of Physicians and Surgeons

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241 Citations (Scopus)

Résumé

Addition of the potent tumor promoter, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), to murine erythroleukemia cell lines in suspension cultures inhibited both spontaneous differentiation and differentiation induced by hexamethylene bisacetamide (HMBA), dimethyl sulfoxide, or butyric acid. Inhibition was unrelated to cytotoxicity and was reversible. When several plant diterpenes were tested, there was a positive correlation between tumor-promoting activity and inhibition of differentiation. TPA inhibited HMBA-induced differentiation only if added prior to the time of commitment to differentiation, as assayed by scoring for differentiation after transfer of cells from HMBA to fresh medium without HMBA. TPA-mediated inhibition of differentiation was associated with a decrease in globin mRNA accumulation.

Langue d'origine	English
Pages (de-à)	3451-3455
Nombre de pages	5
Journal	Unknown Journal
Volume	74
Numéro de publication	8
DOI	https://doi.org/10.1073/pnas.74.8.3451
Statut de publication	Published - 1977

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10.1073/pnas.74.8.3451

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Yamasaki, H., Fibach, E., Nudel, U., Weinstein, I. B., Rifkind, R. A., & Marks, P. A. (1977). Tumor promoters inhibit spontaneous and induced differentiation of murine erythroleukemia cells in culture. Unknown Journal, 74(8), 3451-3455. https://doi.org/10.1073/pnas.74.8.3451

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abstract = "Addition of the potent tumor promoter, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), to murine erythroleukemia cell lines in suspension cultures inhibited both spontaneous differentiation and differentiation induced by hexamethylene bisacetamide (HMBA), dimethyl sulfoxide, or butyric acid. Inhibition was unrelated to cytotoxicity and was reversible. When several plant diterpenes were tested, there was a positive correlation between tumor-promoting activity and inhibition of differentiation. TPA inhibited HMBA-induced differentiation only if added prior to the time of commitment to differentiation, as assayed by scoring for differentiation after transfer of cells from HMBA to fresh medium without HMBA. TPA-mediated inhibition of differentiation was associated with a decrease in globin mRNA accumulation.",

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AU - Yamasaki, H.

AU - Fibach, E.

AU - Nudel, U.

AU - Weinstein, I. B.

AU - Rifkind, R. A.

AU - Marks, P. A.

PY - 1977

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AB - Addition of the potent tumor promoter, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), to murine erythroleukemia cell lines in suspension cultures inhibited both spontaneous differentiation and differentiation induced by hexamethylene bisacetamide (HMBA), dimethyl sulfoxide, or butyric acid. Inhibition was unrelated to cytotoxicity and was reversible. When several plant diterpenes were tested, there was a positive correlation between tumor-promoting activity and inhibition of differentiation. TPA inhibited HMBA-induced differentiation only if added prior to the time of commitment to differentiation, as assayed by scoring for differentiation after transfer of cells from HMBA to fresh medium without HMBA. TPA-mediated inhibition of differentiation was associated with a decrease in globin mRNA accumulation.

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Tumor promoters inhibit spontaneous and induced differentiation of murine erythroleukemia cells in culture

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