An immunogenetically defined and immunodominant Trypanosoma cruzi antigen

R. Ramos; M. Juri; A. Ramos; G. Hoecker; S. Lavandero; P. Pena; A. Morello; Y. Repetto; J. C. Aguillon; A. Ferreira

doi:10.4269/ajtmh.1991.44.314

An immunogenetically defined and immunodominant Trypanosoma cruzi antigen

R. Ramos, M. Juri, A. Ramos, G. Hoecker, S. Lavandero, P. Pena, A. Morello, Y. Repetto, J. C. Aguillon, A. Ferreira

Universidad de Chile

Résultat de recherche › examen par les pairs

22 Citations (Scopus)

Résumé

Two strains of mice, A. SW (H-2(s)) and A.CA (H-2(f)), were immunized with live trypomastigotes or epimastigotes of the Tulahuen strain of Trypanosoma cruzi or with their sonicates. By immunowestern blotting, sera from A.SW mice, but not from A.CA, recognized, in an immunodominant fashion, a 45 kDal polypeptide (Tc45) present in both epimastigotes and trypomastigotes. Since A.SW and A.CA strains are congenic for the major histocompatibility H-2 complex, recognition of Tc45 seems to be controlled by this genetic region or by gene(s) located in its immediate vicinity. Subcellular fractionation revealed that Tc45 is mainly present at the cytoplasmic compartment.

Langue d'origine	English
Pages (de-à)	314-322
Nombre de pages	9
Journal	American Journal of Tropical Medicine and Hygiene
Volume	44
Numéro de publication	3
DOI	https://doi.org/10.4269/ajtmh.1991.44.314
Statut de publication	Published - 1991

ASJC Scopus Subject Areas

Parasitology
Virology
Infectious Diseases

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10.4269/ajtmh.1991.44.314

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title = "An immunogenetically defined and immunodominant Trypanosoma cruzi antigen",

abstract = "Two strains of mice, A. SW (H-2(s)) and A.CA (H-2(f)), were immunized with live trypomastigotes or epimastigotes of the Tulahuen strain of Trypanosoma cruzi or with their sonicates. By immunowestern blotting, sera from A.SW mice, but not from A.CA, recognized, in an immunodominant fashion, a 45 kDal polypeptide (Tc45) present in both epimastigotes and trypomastigotes. Since A.SW and A.CA strains are congenic for the major histocompatibility H-2 complex, recognition of Tc45 seems to be controlled by this genetic region or by gene(s) located in its immediate vicinity. Subcellular fractionation revealed that Tc45 is mainly present at the cytoplasmic compartment.",

author = "R. Ramos and M. Juri and A. Ramos and G. Hoecker and S. Lavandero and P. Pena and A. Morello and Y. Repetto and Aguillon, {J. C.} and A. Ferreira",

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T1 - An immunogenetically defined and immunodominant Trypanosoma cruzi antigen

AU - Ramos, R.

AU - Juri, M.

AU - Ramos, A.

AU - Hoecker, G.

AU - Lavandero, S.

AU - Pena, P.

AU - Morello, A.

AU - Repetto, Y.

AU - Aguillon, J. C.

AU - Ferreira, A.

PY - 1991

Y1 - 1991

N2 - Two strains of mice, A. SW (H-2(s)) and A.CA (H-2(f)), were immunized with live trypomastigotes or epimastigotes of the Tulahuen strain of Trypanosoma cruzi or with their sonicates. By immunowestern blotting, sera from A.SW mice, but not from A.CA, recognized, in an immunodominant fashion, a 45 kDal polypeptide (Tc45) present in both epimastigotes and trypomastigotes. Since A.SW and A.CA strains are congenic for the major histocompatibility H-2 complex, recognition of Tc45 seems to be controlled by this genetic region or by gene(s) located in its immediate vicinity. Subcellular fractionation revealed that Tc45 is mainly present at the cytoplasmic compartment.

AB - Two strains of mice, A. SW (H-2(s)) and A.CA (H-2(f)), were immunized with live trypomastigotes or epimastigotes of the Tulahuen strain of Trypanosoma cruzi or with their sonicates. By immunowestern blotting, sera from A.SW mice, but not from A.CA, recognized, in an immunodominant fashion, a 45 kDal polypeptide (Tc45) present in both epimastigotes and trypomastigotes. Since A.SW and A.CA strains are congenic for the major histocompatibility H-2 complex, recognition of Tc45 seems to be controlled by this genetic region or by gene(s) located in its immediate vicinity. Subcellular fractionation revealed that Tc45 is mainly present at the cytoplasmic compartment.

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An immunogenetically defined and immunodominant Trypanosoma cruzi antigen

Résumé

ASJC Scopus Subject Areas

ODD de l’ONU

Accès au document

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