Osmotically-induced genes are controlled by the transcription factor TonEBP in cultured cardiomyocytes

Paola Navarro; Mario Chiong; Karen Volkwein; Francisco Moraga; María Paz Ocaranza; Jorge E. Jalil; Sun Woo Lim; Jeong Ah Kim; H. Moo Kwon; Sergio Lavandero

doi:10.1016/j.bbrc.2008.05.067

Osmotically-induced genes are controlled by the transcription factor TonEBP in cultured cardiomyocytes

Paola Navarro, Mario Chiong, Karen Volkwein, Francisco Moraga, María Paz Ocaranza, Jorge E. Jalil, Sun Woo Lim, Jeong Ah Kim, H. Moo Kwon, Sergio Lavandero

Universidad de Chile

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Changes in cardiac osmolarity occur in myocardial infarction. Osmoregulatory mechanisms may, therefore, play a crucial role in cardiomyocyte survival. Tonicity-responsive enhancer binding protein (TonEBP) is a key transcription factor participating in the adaptation of cells to increases in tonicity. However, it is unknown whether cardiac TonEBP is activated by tonicity. Hypertonicity activated transcriptional activity of TonEBP, increased the amounts of both TonEBP mRNA and protein, and induced both the mRNA and protein of TonEBP target genes (aldose reductase and heat shock protein-70). Hypotonicity decreased the amount of TonEBP protein indicating bidirectional osmoregulation of this transcription factor. Adenoviral expression of a dominant negative TonEBP suppressed the hypertonicity-dependent increase of aldose reductase protein. These results indicated that TonEBP controls osmoregulatory mechanisms in cardiomyocytes.

Original language	English
Pages (from-to)	326-330
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	372
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2008.05.067
Publication status	Published - Jul 25 2008

Bibliographical note

Funding Information:
We thank Dr. Chris Pogson for his critical reading of the manuscript and Fidel Albornoz for his technical assistance. Funded by Comision Nacional de Ciencia y Tecnología (CONICYT)—Chile (FONDAP 15010006 to S.L.); NIH DK42479 (to H.M.K.). F.M. holds a Ph.D. fellowship from CONICYT, Chile.

Funding

We thank Dr. Chris Pogson for his critical reading of the manuscript and Fidel Albornoz for his technical assistance. Funded by Comision Nacional de Ciencia y Tecnología (CONICYT)—Chile (FONDAP 15010006 to S.L.); NIH DK42479 (to H.M.K.). F.M. holds a Ph.D. fellowship from CONICYT, Chile.

Funders	Funder number
Comision Nacional de Ciencia y Tecnología	15010006
National Institutes of Health
National Institute of Diabetes and Digestive and Kidney Diseases	R01DK042479

ASJC Scopus Subject Areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2008.05.067

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title = "Osmotically-induced genes are controlled by the transcription factor TonEBP in cultured cardiomyocytes",

abstract = "Changes in cardiac osmolarity occur in myocardial infarction. Osmoregulatory mechanisms may, therefore, play a crucial role in cardiomyocyte survival. Tonicity-responsive enhancer binding protein (TonEBP) is a key transcription factor participating in the adaptation of cells to increases in tonicity. However, it is unknown whether cardiac TonEBP is activated by tonicity. Hypertonicity activated transcriptional activity of TonEBP, increased the amounts of both TonEBP mRNA and protein, and induced both the mRNA and protein of TonEBP target genes (aldose reductase and heat shock protein-70). Hypotonicity decreased the amount of TonEBP protein indicating bidirectional osmoregulation of this transcription factor. Adenoviral expression of a dominant negative TonEBP suppressed the hypertonicity-dependent increase of aldose reductase protein. These results indicated that TonEBP controls osmoregulatory mechanisms in cardiomyocytes.",

author = "Paola Navarro and Mario Chiong and Karen Volkwein and Francisco Moraga and Ocaranza, {Mar{\'i}a Paz} and Jalil, {Jorge E.} and Lim, {Sun Woo} and Kim, {Jeong Ah} and Kwon, {H. Moo} and Sergio Lavandero",

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AU - Ocaranza, María Paz

AU - Jalil, Jorge E.

AU - Lim, Sun Woo

AU - Kim, Jeong Ah

AU - Kwon, H. Moo

AU - Lavandero, Sergio

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N2 - Changes in cardiac osmolarity occur in myocardial infarction. Osmoregulatory mechanisms may, therefore, play a crucial role in cardiomyocyte survival. Tonicity-responsive enhancer binding protein (TonEBP) is a key transcription factor participating in the adaptation of cells to increases in tonicity. However, it is unknown whether cardiac TonEBP is activated by tonicity. Hypertonicity activated transcriptional activity of TonEBP, increased the amounts of both TonEBP mRNA and protein, and induced both the mRNA and protein of TonEBP target genes (aldose reductase and heat shock protein-70). Hypotonicity decreased the amount of TonEBP protein indicating bidirectional osmoregulation of this transcription factor. Adenoviral expression of a dominant negative TonEBP suppressed the hypertonicity-dependent increase of aldose reductase protein. These results indicated that TonEBP controls osmoregulatory mechanisms in cardiomyocytes.

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Osmotically-induced genes are controlled by the transcription factor TonEBP in cultured cardiomyocytes

Abstract

Bibliographical note

Funding

ASJC Scopus Subject Areas

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