Osmotically-induced genes are controlled by the transcription factor TonEBP in cultured cardiomyocytes

Paola Navarro; Mario Chiong; Karen Volkwein; Francisco Moraga; María Paz Ocaranza; Jorge E. Jalil; Sun Woo Lim; Jeong Ah Kim; H. Moo Kwon; Sergio Lavandero

doi:10.1016/j.bbrc.2008.05.067

Osmotically-induced genes are controlled by the transcription factor TonEBP in cultured cardiomyocytes

Paola Navarro, Mario Chiong, Karen Volkwein, Francisco Moraga, María Paz Ocaranza, Jorge E. Jalil, Sun Woo Lim, Jeong Ah Kim, H. Moo Kwon, Sergio Lavandero

Universidad de Chile

Résultat de recherche › examen par les pairs

12 Citations (Scopus)

Résumé

Changes in cardiac osmolarity occur in myocardial infarction. Osmoregulatory mechanisms may, therefore, play a crucial role in cardiomyocyte survival. Tonicity-responsive enhancer binding protein (TonEBP) is a key transcription factor participating in the adaptation of cells to increases in tonicity. However, it is unknown whether cardiac TonEBP is activated by tonicity. Hypertonicity activated transcriptional activity of TonEBP, increased the amounts of both TonEBP mRNA and protein, and induced both the mRNA and protein of TonEBP target genes (aldose reductase and heat shock protein-70). Hypotonicity decreased the amount of TonEBP protein indicating bidirectional osmoregulation of this transcription factor. Adenoviral expression of a dominant negative TonEBP suppressed the hypertonicity-dependent increase of aldose reductase protein. These results indicated that TonEBP controls osmoregulatory mechanisms in cardiomyocytes.

Langue d'origine	English
Pages (de-à)	326-330
Nombre de pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	372
Numéro de publication	2
DOI	https://doi.org/10.1016/j.bbrc.2008.05.067
Statut de publication	Published - juill. 25 2008

Financement

We thank Dr. Chris Pogson for his critical reading of the manuscript and Fidel Albornoz for his technical assistance. Funded by Comision Nacional de Ciencia y Tecnología (CONICYT)—Chile (FONDAP 15010006 to S.L.); NIH DK42479 (to H.M.K.). F.M. holds a Ph.D. fellowship from CONICYT, Chile.

Bailleurs de fonds	Numéro du bailleur de fonds
Comision Nacional de Ciencia y Tecnología	15010006
National Institutes of Health
National Institute of Diabetes and Digestive and Kidney Diseases	R01DK042479

ASJC Scopus Subject Areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2008.05.067

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title = "Osmotically-induced genes are controlled by the transcription factor TonEBP in cultured cardiomyocytes",

abstract = "Changes in cardiac osmolarity occur in myocardial infarction. Osmoregulatory mechanisms may, therefore, play a crucial role in cardiomyocyte survival. Tonicity-responsive enhancer binding protein (TonEBP) is a key transcription factor participating in the adaptation of cells to increases in tonicity. However, it is unknown whether cardiac TonEBP is activated by tonicity. Hypertonicity activated transcriptional activity of TonEBP, increased the amounts of both TonEBP mRNA and protein, and induced both the mRNA and protein of TonEBP target genes (aldose reductase and heat shock protein-70). Hypotonicity decreased the amount of TonEBP protein indicating bidirectional osmoregulation of this transcription factor. Adenoviral expression of a dominant negative TonEBP suppressed the hypertonicity-dependent increase of aldose reductase protein. These results indicated that TonEBP controls osmoregulatory mechanisms in cardiomyocytes.",

author = "Paola Navarro and Mario Chiong and Karen Volkwein and Francisco Moraga and Ocaranza, {Mar{\'i}a Paz} and Jalil, {Jorge E.} and Lim, {Sun Woo} and Kim, {Jeong Ah} and Kwon, {H. Moo} and Sergio Lavandero",

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AU - Ocaranza, María Paz

AU - Jalil, Jorge E.

AU - Lim, Sun Woo

AU - Kim, Jeong Ah

AU - Kwon, H. Moo

AU - Lavandero, Sergio

N1 - Funding Information: We thank Dr. Chris Pogson for his critical reading of the manuscript and Fidel Albornoz for his technical assistance. Funded by Comision Nacional de Ciencia y Tecnología (CONICYT)—Chile (FONDAP 15010006 to S.L.); NIH DK42479 (to H.M.K.). F.M. holds a Ph.D. fellowship from CONICYT, Chile.

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N2 - Changes in cardiac osmolarity occur in myocardial infarction. Osmoregulatory mechanisms may, therefore, play a crucial role in cardiomyocyte survival. Tonicity-responsive enhancer binding protein (TonEBP) is a key transcription factor participating in the adaptation of cells to increases in tonicity. However, it is unknown whether cardiac TonEBP is activated by tonicity. Hypertonicity activated transcriptional activity of TonEBP, increased the amounts of both TonEBP mRNA and protein, and induced both the mRNA and protein of TonEBP target genes (aldose reductase and heat shock protein-70). Hypotonicity decreased the amount of TonEBP protein indicating bidirectional osmoregulation of this transcription factor. Adenoviral expression of a dominant negative TonEBP suppressed the hypertonicity-dependent increase of aldose reductase protein. These results indicated that TonEBP controls osmoregulatory mechanisms in cardiomyocytes.

AB - Changes in cardiac osmolarity occur in myocardial infarction. Osmoregulatory mechanisms may, therefore, play a crucial role in cardiomyocyte survival. Tonicity-responsive enhancer binding protein (TonEBP) is a key transcription factor participating in the adaptation of cells to increases in tonicity. However, it is unknown whether cardiac TonEBP is activated by tonicity. Hypertonicity activated transcriptional activity of TonEBP, increased the amounts of both TonEBP mRNA and protein, and induced both the mRNA and protein of TonEBP target genes (aldose reductase and heat shock protein-70). Hypotonicity decreased the amount of TonEBP protein indicating bidirectional osmoregulation of this transcription factor. Adenoviral expression of a dominant negative TonEBP suppressed the hypertonicity-dependent increase of aldose reductase protein. These results indicated that TonEBP controls osmoregulatory mechanisms in cardiomyocytes.

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Osmotically-induced genes are controlled by the transcription factor TonEBP in cultured cardiomyocytes

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